≡

ELISA Kit for Methyl CpG Binding Protein 2 (MECP2)

RTS; AUTSX3; MRX16; MRX79; PPMX; RTT; Rett Syndrome; Mental Retardation,X-linked 16

Specificity

This assay has high sensitivity and excellent specificity for detection of Methyl CpG Binding Protein 2 (MECP2).
No significant cross-reactivity or interference between Methyl CpG Binding Protein 2 (MECP2) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Methyl CpG Binding Protein 2 (MECP2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Methyl CpG Binding Protein 2 (MECP2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Scientific Reports An electrochemiluminescence based assay for quantitative detection of endogenous and exogenously applied MeCP2 protein variants Pubmed: 31138832
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPC616Hu01 Recombinant Methyl CpG Binding Protein 2 (MECP2) Positive Control; Immunogen; SDS-PAGE; WB.
PAC616Hu01 Polyclonal Antibody to Methyl CpG Binding Protein 2 (MECP2) WB; IHC; ICC; IP.
MAC616Hu21 Monoclonal Antibody to Methyl CpG Binding Protein 2 (MECP2) WB; IHC; ICC; IP.
MAC616Hu22 Monoclonal Antibody to Methyl CpG Binding Protein 2 (MECP2) WB; IHC; ICC; IP.
MAC616Hu23 Monoclonal Antibody to Methyl CpG Binding Protein 2 (MECP2) WB
MAC616Hu24 Monoclonal Antibody to Methyl CpG Binding Protein 2 (MECP2) WB; IHC; ICC; IP.
SEC616Hu ELISA Kit for Methyl CpG Binding Protein 2 (MECP2) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC616Hu Multiplex Assay Kit for Methyl CpG Binding Protein 2 (MECP2) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.