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ELISA Kit for Afamin (AFM)
ALB2; ALBA; ALF; A-ALB; Alpha-Albumin
- Product No.SEC284Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range3.12-200ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 1.45ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 490
For more details, please contact local distributors! US$ 700 US$ 3150 US$ 5950 US$ 49000
Specificity
This assay has high sensitivity and excellent specificity for detection of Afamin (AFM).
No significant cross-reactivity or interference between Afamin (AFM) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Afamin (AFM) and the recovery rates were calculated by comparing the measured value to the expected amount of Afamin (AFM) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 85-99 | 91 |
EDTA plasma(n=5) | 80-96 | 90 |
heparin plasma(n=5) | 89-102 | 97 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Afamin (AFM) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Afamin (AFM) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Afamin (AFM) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 79-95% | 79-96% | 92-101% | 89-101% |
EDTA plasma(n=5) | 91-99% | 89-101% | 80-93% | 97-104% |
heparin plasma(n=5) | 97-105% | 85-99% | 81-102% | 78-91% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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Magazine | Citations |
Journal of Proteomics | 2D-DIGE analysis of sera from transgenic mouse models reveals novel candidate protein biomarkers for human gastric cancer ScienceDirect: S1874391912004964 |
Clin Chim Acta | Afamin — A pleiotropic glycoprotein involved in various disease states PubMed: 25892677 |
Nutrients | Gene Expression of Sirtuin-1 and Endogenous Secretory Receptor for Advanced Glycation End Products in Healthy and Slightly Overweight Subjects after … Pubmed:30037068 |
Biomarkers in Medicine | Urine afamin and afamin–creatinine ratio as biomarkers for kidney injury Pubmed: 30433819 |
Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
RPC284Hu01 | Recombinant Afamin (AFM) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPC284Hu02 | Recombinant Afamin (AFM) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAC284Hu01 | Polyclonal Antibody to Afamin (AFM) | WB; IHC; ICC; IP. |
PAC284Hu02 | Polyclonal Antibody to Afamin (AFM) | WB; IHC; ICC; IP. |
LAC284Hu71 | Biotin-Linked Polyclonal Antibody to Afamin (AFM) | WB; IHC; ICC. |
MAC284Hu22 | Monoclonal Antibody to Afamin (AFM) | WB; IHC; ICC; IP. |
SEC284Hu | ELISA Kit for Afamin (AFM) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMC284Hu | Multiplex Assay Kit for Afamin (AFM) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
KSC284Hu01 | ELISA Kit DIY Materials for Afamin (AFM) | Main materials for "Do It (ELISA Kit) Yourself". |