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ELISA Kit for Superoxide Dismutase 1 (SOD1)
ALS; ALS1; IPOA; Superoxide Dismutase 1, Soluble; Amyotrophic Lateral Sclerosis 1,Adult; Superoxide dismutase [Cu-Zn]
- Product No.SEB960Ga
- Organism SpeciesChicken (Gallus) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenatesand other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range31.2-2,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 13.3pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 505
For more details, please contact local distributors! US$ 722 US$ 3249 US$ 6137 US$ 50540
Specificity
This assay has high sensitivity and excellent specificity for detection of Superoxide Dismutase 1 (SOD1).
No significant cross-reactivity or interference between Superoxide Dismutase 1 (SOD1) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Superoxide Dismutase 1 (SOD1) and the recovery rates were calculated by comparing the measured value to the expected amount of Superoxide Dismutase 1 (SOD1) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 89-97 | 94 |
EDTA plasma(n=5) | 89-103 | 92 |
heparin plasma(n=5) | 78-90 | 84 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Superoxide Dismutase 1 (SOD1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Superoxide Dismutase 1 (SOD1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Superoxide Dismutase 1 (SOD1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 92-103% | 90-99% | 80-101% | 96-105% |
EDTA plasma(n=5) | 87-95% | 88-102% | 94-103% | 94-103% |
heparin plasma(n=5) | 91-98% | 95-104% | 93-105% | 83-102% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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Magazine | Citations |
AGE | Consequences of age on ischemic wound healing in rats: altered antioxidant activity and delayed wound closure Pubmed:24443098 |
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Arch Oral Biol | Antioxidant profile, carbonyl and lipid oxidation markers in the parotid and submandibular glands of rats in different periods of streptozotocin induced diabetes PubMed: 26143097 |
Oxidative Medicine and Cellular Longevity | BQ123 Stimulates Skeletal Muscle Antioxidant Defense via Nrf2 Activation in LPS-Treated Rats Journals: Omcl |
О Р И Г И Н А Л Ь Н Ы Е С Т А Т Ь И | КЛИНИЧЕСКОЕ ЗНАЧЕНИЕ ФАКТОРОВ КЛЕТОЧНОЙ РЕГУЛЯЦИИ И ФЕРМЕНТОВ АНТИОКСИДАНТНОЙ СИСТЕМЫ У ПАЦИЕНТОК РЕПРОДУКТИВНОГО ВОЗРАСТА С НАРУЖНЫМ ГЕНИТАЛЬНЫМ ЭНДОМЕТРИ docs:225188 |
Evidence-Based Complementary and Alternative Medicine | Metabolomic Analysis of Biochemical Changes in the Plasma of High-Fat Diet and Streptozotocin-Induced Diabetic Rats after Treatment with Isoflavones Extract of Radix Puerariae pmc:PMC4794592 |
Free Radic Biol Med. | DDAH1 deficiency promotes intracellular oxidative stress and cell apoptosis via a miR-21-dependent pathway in mouse embryonic fibroblasts. Pubmed:26806551 |
Adv Med Sci. | Orthodontic treatment modifies the oxidant-antioxidant balance in saliva of clinically healthy subjects. pubmed:28242484 |
Iranian Journal of Kidney Diseases | Clinical Characterization of Serum Docosahexaenoic Acid and Its Relationship With Inflammation Factors in Patients With Diabetic Nephropathy Pubmed:29507271 |
Journal of Integrative Medicine | Effects of Tualang honey in modulating nociceptive responses at the spinal cord in offspring of prenatally stressed rats Doi: 10.1016/j.joim.2018.12.002 |
Metallomics | Restoration of myocellular copper-trafficking proteins and mitochondrial copper enzymes repairs cardiac function in rats with diabetes-evoked heart failure Pubmed: 31821401 |
JOURNAL OF PHARMACY AND PHARMACOLOGY | Grape seed extract improved the fertility-enhancing effect of atorvastatin in high-fat diet-induced testicular injury in rats: involvement of antioxidant and anti-apoptotic?¡ 33793875 |
ACS Omega | SOD1 is a Possible Predictor of COVID-19 Progression as Revealed by Plasma Proteomics 34250342 |
Korean J Physiol Pharmacol | Improved motility in the gastrointestinal tract of a postoperative ileus rat model with ilaprazole 34697261 |
Pharmaceuticals | Serum Total SOD Activity and SOD1/2 Concentrations in Predicting All-Cause Mortality in Lung Cancer Patients 34832849 |
Catalog No. | Related products for research use of Chicken (Gallus) Organism species | Applications (RESEARCH USE ONLY!) |
RPB960Ga01 | Recombinant Superoxide Dismutase 1 (SOD1) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB960Ga01 | Polyclonal Antibody to Superoxide Dismutase 1 (SOD1) | WB; IHC; ICC; IP. |
SEB960Ga | ELISA Kit for Superoxide Dismutase 1 (SOD1) | Enzyme-linked immunosorbent assay for Antigen Detection. |