ELISA Kit for Cluster Of Differentiation 147 (CD147)

EMMPRIN; BSG; 5F7; M6; OK; TCSF; Basigin(Ok Blood Group); Collagenase stimulatory factor; OK blood group antigen; Tumor cell-derived collagenase stimulatory factor

Specificity

This assay has high sensitivity and excellent specificity for detection of Cluster Of Differentiation 147 (CD147).
No significant cross-reactivity or interference between Cluster Of Differentiation 147 (CD147) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Cluster Of Differentiation 147 (CD147) and the recovery rates were calculated by comparing the measured value to the expected amount of Cluster Of Differentiation 147 (CD147) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 87-101 98
EDTA plasma(n=5) 96-105 99
heparin plasma(n=5) 80-99 88

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cluster Of Differentiation 147 (CD147) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cluster Of Differentiation 147 (CD147) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cluster Of Differentiation 147 (CD147) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 78-99% 78-94% 87-101% 93-102%
EDTA plasma(n=5) 90-104% 84-94% 93-101% 87-99%
heparin plasma(n=5) 85-98% 97-104% 89-102% 98-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
DISEASE MARKERS The Combination of CD147 and MMP-9 Serum Levels Is Identified as Novel Chemotherapy Response Markers of Advanced Non-Small-Cell Lung Cancer Pubmed: 32377273
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APB540Hu61 Active Cluster Of Differentiation 147 (CD147) Cell culture; Activity Assays.
RPB540Hu01 Recombinant Cluster Of Differentiation 147 (CD147) Positive Control; Immunogen; SDS-PAGE; WB.
RPB540Hu02 Recombinant Cluster Of Differentiation 147 (CD147) Positive Control; Immunogen; SDS-PAGE; WB.
EPB540Hu61 Eukaryotic Cluster Of Differentiation 147 (CD147) Positive Control; Immunogen; SDS-PAGE; WB.
APB540Hu01 Active Cluster Of Differentiation 147 (CD147) Cell culture; Activity Assays.
APB540Hu02 Active Cluster Of Differentiation 147 (CD147) Cell culture; Activity Assays.
PAB540Hu01 Polyclonal Antibody to Cluster Of Differentiation 147 (CD147) WB; IHC; ICC; IP.
FAB540Hu01 Anti-Cluster Of Differentiation 147 (CD147) Polyclonal Antibody Flow cytometry.
MAB540Hu22 Monoclonal Antibody to Cluster Of Differentiation 147 (CD147) WB; IHC; ICC; IP.
MAB540Hu21 Monoclonal Antibody to Cluster Of Differentiation 147 (CD147) WB; IHC; ICC; IP.
MAB540Hu27 Monoclonal Antibody to Cluster Of Differentiation 147 (CD147) WB; IHC; ICC; IP.
MAB540Hu24 Monoclonal Antibody to Cluster Of Differentiation 147 (CD147) WB; IHC; ICC; IP.
MAB540Hu25 Monoclonal Antibody to Cluster Of Differentiation 147 (CD147) WB; IHC; ICC; IP.
MAB540Hu23 Monoclonal Antibody to Cluster Of Differentiation 147 (CD147) WB; IHC; ICC; IP.
MAB540Hu28 Monoclonal Antibody to Cluster Of Differentiation 147 (CD147) WB; IHC; ICC; IP.
MAB540Hu29 Monoclonal Antibody to Cluster Of Differentiation 147 (CD147) WB; IHC; ICC; IP.
FAB540Hu02 Anti-Cluster Of Differentiation 147 (CD147) Monoclonal Antibody FCM
SEB540Hu ELISA Kit for Cluster Of Differentiation 147 (CD147) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB540Hu Multiplex Assay Kit for Cluster Of Differentiation 147 (CD147) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.