≡

ELISA Kit for Cytokeratin 19 (CK19)

K19; KRT19; K1CS; Keratin 19; 40-kDa Keratin Intermediate Filament; Keratin, type I cytoskeletal 19

Specificity

This assay has high sensitivity and excellent specificity for detection of Cytokeratin 19 (CK19).
No significant cross-reactivity or interference between Cytokeratin 19 (CK19) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Cytokeratin 19 (CK19) and the recovery rates were calculated by comparing the measured value to the expected amount of Cytokeratin 19 (CK19) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 91-98 94
EDTA plasma(n=5) 78-102 81
heparin plasma(n=5) 98-105 102

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytokeratin 19 (CK19) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytokeratin 19 (CK19) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cytokeratin 19 (CK19) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-95% 80-99% 91-98% 94-102%
EDTA plasma(n=5) 85-99% 99-105% 92-105% 97-105%
heparin plasma(n=5) 84-98% 98-105% 81-96% 91-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPB239Mu02 Recombinant Cytokeratin 19 (CK19) Positive Control; Immunogen; SDS-PAGE; WB.
RPB239Mu01 Recombinant Cytokeratin 19 (CK19) Positive Control; Immunogen; SDS-PAGE; WB.
PAB239Mu02 Polyclonal Antibody to Cytokeratin 19 (CK19) WB; IHC; ICC; IP.
PAB239Mu01 Polyclonal Antibody to Cytokeratin 19 (CK19) WB; IHC; ICC; IP.
LAB239Mu81 FITC-Linked Polyclonal Antibody to Cytokeratin 19 (CK19) WB; IHC; ICC; IF.
LAB239Mu71 Biotin-Linked Polyclonal Antibody to Cytokeratin 19 (CK19) WB; IHC; ICC.
SEB239Mu ELISA Kit for Cytokeratin 19 (CK19) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB239Mu Multiplex Assay Kit for Cytokeratin 19 (CK19) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB239Mu01 ELISA Kit DIY Materials for Cytokeratin 19 (CK19) Main materials for "Do It(ELISA Kit) Yourself"