ELISA Kit for L1-Cell Adhesion Molecule (L1CAM)

CD171; L1-CAM; CAML1; HSAS; HSAS1; MASA; MIC5; N-CAML1; S10; SPG1; Antigen Identified By Monoclonal Antibody R1

Specificity

This assay has high sensitivity and excellent specificity for detection of L1-Cell Adhesion Molecule (L1CAM).
No significant cross-reactivity or interference between L1-Cell Adhesion Molecule (L1CAM) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant L1-Cell Adhesion Molecule (L1CAM) and the recovery rates were calculated by comparing the measured value to the expected amount of L1-Cell Adhesion Molecule (L1CAM) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 97-105 101
EDTA plasma(n=5) 86-101 97
heparin plasma(n=5) 97-105 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level L1-Cell Adhesion Molecule (L1CAM) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level L1-Cell Adhesion Molecule (L1CAM) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of L1-Cell Adhesion Molecule (L1CAM) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 81-97% 96-103% 84-94% 81-105%
EDTA plasma(n=5) 90-105% 79-88% 85-101% 92-102%
heparin plasma(n=5) 86-101% 88-101% 78-92% 95-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
Arch Gynecol Obstet. The sL1CAM in sera of patients with endometrial and ovarian cancers. pubmed:27832351
Journal of Neurotrauma L1CAM overexpression downregulates phosphacan and upregulates structural plasticity-related genes rostral and caudal to the complete spinal cord transection Pubmed: 31426714
Cancers Extracellular Vesicles-Based Biomarkers Represent a Promising Liquid Biopsy in Endometrial Cancer Pubmed: 31842290
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