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ELISA Kit for Coagulation Factor V (F5)
FV; FVL; PCCF; Proaccelerin; Labile Factor; Factor V Leiden; Accelerator Globulin; Activated protein C cofactor; Proaccelerin, labile factor
- Product No.SEA675Bo
- Organism SpeciesBos taurus; Bovine (Cattle) Same name, Different species.
- Sample Typeserum, plasma and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range15.6-1,000ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 5.5ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 588
For more details, please contact local distributors! US$ 840 US$ 3780 US$ 7140 US$ 58800
Specificity
This assay has high sensitivity and excellent specificity for detection of Coagulation Factor V (F5).
No significant cross-reactivity or interference between Coagulation Factor V (F5) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Coagulation Factor V (F5) and the recovery rates were calculated by comparing the measured value to the expected amount of Coagulation Factor V (F5) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 96-105 | 101 |
EDTA plasma(n=5) | 93-105 | 101 |
heparin plasma(n=5) | 87-94 | 91 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Coagulation Factor V (F5) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Coagulation Factor V (F5) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Coagulation Factor V (F5) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 96-103% | 95-103% | 83-103% | 79-101% |
EDTA plasma(n=5) | 80-99% | 89-103% | 86-101% | 97-105% |
heparin plasma(n=5) | 82-104% | 78-89% | 90-101% | 89-104% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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Magazine | Citations |
Biochem Biophys Res Commun | Thrombin generation in abdominal sepsis is Rho-kinase-dependent PubMed: 25813486 |
Cell Transplantation | Evidence of a Pivotal Role for the Distal Part of the Complement Cascade in the Diurnal Release of Hematopoietic Stem Cells Into Peripheral Blood Pubmed:26087465 |
Cardiovascular Research | Loss of accelerates arterial thrombosis by increasing formation of neutrophil extracellular traps and plasma tissue factor activity Pubmed:29444200 |
Catalog No. | Related products for research use of Bos taurus; Bovine (Cattle) Organism species | Applications (RESEARCH USE ONLY!) |
RPA675Bo02 | Recombinant Coagulation Factor V (F5) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA675Bo01 | Recombinant Coagulation Factor V (F5) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA675Bo02 | Polyclonal Antibody to Coagulation Factor V (F5) | WB; IHC; ICC; IP. |
PAA675Bo01 | Polyclonal Antibody to Coagulation Factor V (F5) | WB; IHC; ICC; IP. |
SEA675Bo | ELISA Kit for Coagulation Factor V (F5) | Enzyme-linked immunosorbent assay for Antigen Detection. |