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ELISA Kit for Slit Homolog 2 (Slit2)
SLIL3
- Product No.SEA672Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample Typetissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range1.56-100ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.55ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 441
For more details, please contact local distributors! US$ 630 US$ 2835 US$ 5355 US$ 44100
Specificity
This assay has high sensitivity and excellent specificity for detection of Slit Homolog 2 (Slit2).
No significant cross-reactivity or interference between Slit Homolog 2 (Slit2) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Slit Homolog 2 (Slit2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Slit Homolog 2 (Slit2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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Magazine | Citations |
Cancer Research | SLIT2 Attenuation during Lung Cancer Progression Deregulates β-Catenin and E-Cadherin and Associates with Poor Prognosis AACR: 702543 |
Carcinogenesis | Slit-2 facilitates interaction of P-cadherin with Robo-3 and inhibits cell migration in an oral squamous cell carcinoma cell line OxfordJournals: source |
PLoS One | Analytical Performance of ELISA Assays in Urine: One More Bottleneck towards Biomarker Validation and Clinical Implementation Pubmed:26889680 |
ESC Heart Fail | Crosstalk between the activated Slit2¨CRobo1 pathway and TGF©\¦Â1 signalling promotes cardiac fibrosis 33236535 |
Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
RPA672Hu03 | Recombinant Slit Homolog 2 (Slit2) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA672Hu01 | Recombinant Slit Homolog 2 (Slit2) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA672Hu02 | Recombinant Slit Homolog 2 (Slit2) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA672Hu01 | Polyclonal Antibody to Slit Homolog 2 (Slit2) | WB; IHC; ICC; IP. |
PAA672Hu02 | Polyclonal Antibody to Slit Homolog 2 (Slit2) | WB; IHC; ICC; IP. |
PAA672Hu03 | Polyclonal Antibody to Slit Homolog 2 (Slit2) | WB; IHC; ICC; IP. |
LAA672Hu71 | Biotin-Linked Polyclonal Antibody to Slit Homolog 2 (Slit2) | WB; IHC; ICC. |
MAA672Hu22 | Monoclonal Antibody to Slit Homolog 2 (Slit2) | WB; IHC; ICC; IP. |
SEA672Hu | ELISA Kit for Slit Homolog 2 (Slit2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA672Hu | Multiplex Assay Kit for Slit Homolog 2 (Slit2) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |