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ELISA Kit for Matrix Metalloproteinase 15 (MMP15)
SMCP-2; MTMMP2; MT2-MMP; Membrane-Inserted; Membrane-type matrix metalloproteinase 2; Membrane-type-2 matrix metalloproteinase
- Product No.SEA322Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range31.2-2,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 11.2pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 454
For more details, please contact local distributors! US$ 648 US$ 2916 US$ 5508 US$ 45360
Specificity
This assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 15 (MMP15).
No significant cross-reactivity or interference between Matrix Metalloproteinase 15 (MMP15) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Matrix Metalloproteinase 15 (MMP15) and the recovery rates were calculated by comparing the measured value to the expected amount of Matrix Metalloproteinase 15 (MMP15) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 83-103 | 87 |
heparin plasma(n=5) | 91-104 | 97 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 15 (MMP15) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 15 (MMP15) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Matrix Metalloproteinase 15 (MMP15) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 81-91% | 89-103% | 81-88% | 84-98% |
heparin plasma(n=5) | 83-104% | 92-101% | 80-91% | 78-99% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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Magazine | Citations |
Phytomedicine | Asiatic acid from centella asiatica exert anti-invasive ability in human renal cancer cells by modulation of ERK/p38MAPK-mediated MMP15 expression Pubmed:35316724 |
Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
RPA322Mu01 | Recombinant Matrix Metalloproteinase 15 (MMP15) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA322Mu02 | Recombinant Matrix Metalloproteinase 15 (MMP15) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA322Mu01 | Polyclonal Antibody to Matrix Metalloproteinase 15 (MMP15) | WB; IHC; ICC; IP. |
PAA322Mu02 | Polyclonal Antibody to Matrix Metalloproteinase 15 (MMP15) | WB; IHC; ICC; IP. |
SEA322Mu | ELISA Kit for Matrix Metalloproteinase 15 (MMP15) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA322Mu | Multiplex Assay Kit for Matrix Metalloproteinase 15 (MMP15) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |