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ELISA Kit for Galectin 1 (GAL1)

LGALS1; GBP; HLBP14; Galaptin; Lectin,Galactoside-Binding Soluble 1; 14 kDa laminin-binding protein; Lactose-binding lectin 1; Putative MAPK-activating protein PM12; S-Lac lectin

Specificity

This assay has high sensitivity and excellent specificity for detection of Galectin 1 (GAL1).
No significant cross-reactivity or interference between Galectin 1 (GAL1) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Galectin 1 (GAL1) and the recovery rates were calculated by comparing the measured value to the expected amount of Galectin 1 (GAL1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 78-92 87
EDTA plasma(n=5) 98-105 102
heparin plasma(n=5) 89-96 92

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Galectin 1 (GAL1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Galectin 1 (GAL1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Galectin 1 (GAL1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 92-101% 78-104% 90-102% 92-103%
EDTA plasma(n=5) 82-96% 81-96% 89-103% 85-99%
heparin plasma(n=5) 85-95% 82-93% 85-104% 84-93%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
International Journal of Oncology Galectin-1-mediated cell adhesion, invasion and cell death in human anaplastic large cell lymphoma: Regulatory roles of cell surface glycans Pubmed:24589677
Clinica Chimica Acta Galectin-1 and galectin-3: plausible tumour markers for oral squamous cell carcinoma and suitable targets for screening high-risk population Pubmed:25578395
Journal of Perinatal Medicine Cord blood galectin-1 and-3 concentrations in term pregnancies with normal restricted and increased fetal growth PubMed: 24717333
Biomaterials Noninvasive small-animal imaging of galectin-1 upregulation for predicting tumor resistance to radiotherapy Pubmed:29272764
Advances in Experimental Medicine and Biology Molecules of Damage-Associated Patterns in Bronchoalveolar Lavage Fluid and Serum in Chronic Obstructive Pulmonary Disease Pubmed:29429028
RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY Elevated level of Galectin-1 in bronchoalveolar lavage of patients with idiopathic pulmonary fibrosis Pubmed: 31678459
Eur J Nucl Med Mol Imaging Galectin expression detected by 68Ga-galectracer PET as a predictive biomarker of radiotherapy resistance Pubmed:35106644
Medicine Regulatory T cells, damage-associated molecular patterns, and myeloid-derived suppressor cells in bronchoalveolar lavage fluid interlinked with chronic obstructive … Pubmed:35687771
In Vitro Bovine Embryo Production Using Medium's Supplemented with Recombinant Human Galectin-1 Buffered
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