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ELISA Kit for Pyruvate kinase isozymes R/L (PKLR)
PK; PyK; PK1; PKL; Pyruvate Kinase Isozymes R/L; Glutamic--pyruvic transaminase 1; R-type/L-type pyruvate kinase; Red cell/liver pyruvate kinase
- Product No.SEA208Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates and other biological fluids.
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.781-50ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.35ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 479
For more details, please contact local distributors! US$ 684 US$ 3078 US$ 5814 US$ 47880
Specificity
This assay has high sensitivity and excellent specificity for detection of Pyruvate kinase isozymes R/L (PKLR).
No significant cross-reactivity or interference between Pyruvate kinase isozymes R/L (PKLR) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Pyruvate kinase isozymes R/L (PKLR) and the recovery rates were calculated by comparing the measured value to the expected amount of Pyruvate kinase isozymes R/L (PKLR) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 82-97 | 88 |
EDTA plasma(n=5) | 87-98 | 93 |
heparin plasma(n=5) | 94-105 | 98 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pyruvate kinase isozymes R/L (PKLR) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pyruvate kinase isozymes R/L (PKLR) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pyruvate kinase isozymes R/L (PKLR) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 79-89% | 80-92% | 78-102% | 82-90% |
EDTA plasma(n=5) | 89-99% | 96-103% | 96-105% | 96-105% |
heparin plasma(n=5) | 94-105% | 87-95% | 88-104% | 79-89% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
RPA208Ra01 | Recombinant Pyruvate kinase isozymes R/L (PKLR) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA208Ra01 | Polyclonal Antibody to Pyruvate kinase isozymes R/L (PKLR) | WB; IHC; ICC; IP. |
MAA208Ra21 | Monoclonal Antibody to Pyruvate kinase isozymes R/L (PKLR) | WB; IHC; ICC; IP. |
SEA208Ra | ELISA Kit for Pyruvate kinase isozymes R/L (PKLR) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA208Ra | Multiplex Assay Kit for Pyruvate kinase isozymes R/L (PKLR) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |