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ELISA Kit for Neurotrophin 3 (NT3)
NTF3; HDNF; NGF2; Nerve Growth Factor 2; Neurotrophic Factor
- Product No.SEA106Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range3.12-200pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 1.15pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 479
For more details, please contact local distributors! US$ 684 US$ 3078 US$ 5814 US$ 47880
Specificity
This assay has high sensitivity and excellent specificity for detection of Neurotrophin 3 (NT3).
No significant cross-reactivity or interference between Neurotrophin 3 (NT3) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Neurotrophin 3 (NT3) and the recovery rates were calculated by comparing the measured value to the expected amount of Neurotrophin 3 (NT3) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 89-97 | 94 |
EDTA plasma(n=5) | 78-92 | 86 |
heparin plasma(n=5) | 91-99 | 94 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neurotrophin 3 (NT3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neurotrophin 3 (NT3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Neurotrophin 3 (NT3) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 95-105% | 98-105% | 85-93% | 90-97% |
EDTA plasma(n=5) | 82-103% | 83-93% | 91-99% | 96-104% |
heparin plasma(n=5) | 78-96% | 87-105% | 80-90% | 80-94% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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Magazine | Citations |
S | Caracteriza Teses: Disponiveis |
Canadian Journal of Physiology and Pharmacology | Protective effects of telmisartan and tempol on lipopolysaccharide-induced cognitive impairment, neuroinflammation, and amyloidogenesis: possible role of brain-derived neurotrophic factor pubmed:28388365 |
Efecto de Diferentes Intervenciones de Ejercicio Físico Agudo Sobre Niveles Plasmáticos de BDNF, NT-3 y NT-4 en Adultos con Sobrepeso : | |
Artificial Cells Nanomedicine and Biotechnology | Histological and functional outcomes in a rat model of hemisected spinal cord with sustained VEGF/NT-3 release from tissue-engineered grafts Pubmed: 31899965 |
laboratory investigation | Macrophage-derived neurotrophin-3 promotes heterotopic ossification in rats Pubmed: 31896816 |
EUROPEAN CHILD & ADOLESCENT PSYCHIATRY | Increased serum brain-derived neurotrophic factor and adrenocorticotropic hormone levels are associated with obsessive compulsive disorder in medication?free?¡ 33389158 |
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | Sea urchin gangliosides exhibit neuritogenic effects in neuronal PC12 cells via TrkA- and TrkB-related pathways 33589896 |
Clin Epigenetics | Genome?wide profiling of DNA methylation and gene expression unravel the epigenetic landscape in diabetes-related hypothyroidism 34092241 |
J Nutr Sci Vitaminol (Tokyo) | Effects of a Tea Cultivar “MK5601” on Behaviors and Hippocampal Neurotrophin-3 Levels in Middle-Aged Mice 34193676 |
Iranian Journal of Basic Medical Sciences | Adrenomedullin increases cAMP accumulation and BDNF expression in rat DRG and spinal motor neurons 34712429 |
Molecular Biology Reports | Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling … Pubmed:35585377 |
Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
RPA106Ra01 | Recombinant Neurotrophin 3 (NT3) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA106Ra02 | Recombinant Neurotrophin 3 (NT3) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA106Ra01 | Polyclonal Antibody to Neurotrophin 3 (NT3) | WB; IHC; ICC; IP. |
MAA106Ra21 | Monoclonal Antibody to Neurotrophin 3 (NT3) | WB; IHC; ICC; IP. |
SEA106Ra | ELISA Kit for Neurotrophin 3 (NT3) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA106Ra | Multiplex Assay Kit for Neurotrophin 3 (NT3) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |