ELISA Kit for Matrix Metalloproteinase 7 (MMP7)

MPSL1; MAT; PUMP-1; Matrilysin Uterine; Matrilysin; Matrin; Pump-1 Protease; Uterine Metalloproteinase


This assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 7 (MMP7).
No significant cross-reactivity or interference between Matrix Metalloproteinase 7 (MMP7) and analogues was observed.


Matrices listed below were spiked with certain level of recombinant Matrix Metalloproteinase 7 (MMP7) and the recovery rates were calculated by comparing the measured value to the expected amount of Matrix Metalloproteinase 7 (MMP7) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 96-105 101
heparin plasma(n=5) 80-96 86


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 7 (MMP7) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 7 (MMP7) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Matrix Metalloproteinase 7 (MMP7) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 82-97% 88-101% 95-104% 97-105%
heparin plasma(n=5) 83-95% 87-97% 80-102% 96-104%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.



Magazine Citations
British Journal of Pharmacology A novel, orally active LPA1 receptor antagonist inhibits lung fibrosis in the mouse bleomycin model PubMed: 20649573
Neuroimmunomodulation Upregulated Expression of Matrix Metalloproteinases and Tissue Inhibitors of Matrix Metalloproteinases in BALB/c Mouse Brain Challenged with Japanese Encephalitis Virus Pubmed: source
PLoS ONE A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source
Thromb Haemost. Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke Pubmed:24671655
Histol Histopathol Estrogen-deficient osteoporosis enhances the recruitment and activity of osteoclasts by breast cancer cells PubMed: 26254457
VirusDisease Circulating levels of matrix metalloproteinases and tissue inhibitors of matrix metalloproteinases during Japanese encephalitis virus infection Pubmed:26925446
Am J Physiol Lung Cell Mol Physiol Systematic phenotyping and correlation of biomarkers with lung function and histology in lung fibrosis Pubmed:26993522
Cellular Physiology and Biochemistry The MiR-495/Annexin A3/P53 Axis Inhibits the Invasion and EMT of Colorectal Cancer Cells pubmed:29224019
s-space Thyroid disrupting effects and associated mechanisms of TCPP in GH3 cell line and zebrafish (Danio rerio) larva and adult 10371/137708
Science Translational Medicine Large-scale proteomics identifies MMP-7 as a sentinel of epithelial injury and of biliary atresia. pubmed:29167395
Hepatology Diagnostic Accuracy of Serum Matrix Metalloproteinase‐7 for Biliary Atresia Pubmed: 30153340
Journal of Pediatrics Quantification of Serum Matrix Metallopeptide 7 Levels May Assist in the Diagnosis and Predict the Outcome for Patients with Biliary Atresia Pubmed: 30853207
Pediatrics Serum MMP-7 in the Diagnosis of Biliary Atresia Pubmed: 31604829
Biomed Pharmacother Human umbilical cord blood mononuclear cells protect against renal tubulointerstitial fibrosis in cisplatin-treated rats Pubmed: 31710895
LABORATORY INVESTIGATION The synthetic toxin biliatresone causes biliary atresia in mice Pubmed: 32681026
diagnostics Interstitial Score and Concentrations of IL-4R¦Á, PAR-2, and MMP-7 in Bronchoalveolar Lavage Fluid Could Be Useful Markers for Distinguishing Idiopathic Interstitial?¡­ 33924683
Cancer Cell Int A novel strategy to identify candidate diagnostic and prognostic biomarkers for gastric cancer 34215253
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
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