Packages (Simulation)
![CLIA Kit for Tumor Protein, Translationally Controlled 1 (TPT1)](/upload/static/product/SC.jpg)
Reagent Preparation
![CLIA Kit for Tumor Protein, Translationally Controlled 1 (TPT1)](/upload/static/reagent/TSC.jpg)
Image (I)
Image (II)
Certificate
![Certificate](/upload/static/ISO13485.jpg)
![Certificate](/upload/static/ce-1.jpg)
CLIA Kit for Tumor Protein, Translationally Controlled 1 (TPT1)
HRF; TCTP; p02; p23; Fortilin; Translationally-Controlled Tumor Protein; Histamine-releasing factor
- Product No.SCG962Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample Typetissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length2h, 40min
- Detection Range27.4-20,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 10.3pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 605
For more details, please contact local distributors! US$ 864 US$ 3888 US$ 7344 US$ 60480
Specificity
This assay has high sensitivity and excellent specificity for detection of Tumor Protein, Translationally Controlled 1 (TPT1).
No significant cross-reactivity or interference between Tumor Protein, Translationally Controlled 1 (TPT1) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Protein, Translationally Controlled 1 (TPT1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Protein, Translationally Controlled 1 (TPT1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
Substrate A | 1×10mL | Substrate B | 1×2mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.
GIVEAWAYS
INCREMENT SERVICES
-
Single-component Reagents of Assay Kit
-
Lysis Buffer Specific for ELISA / CLIA
-
Quality Control of Kit
-
CLIA Kit Customized Service
-
Disease Model Customized Service
-
Serums Customized Service
-
TGFB1 Activation Reagent
-
Real Time PCR Experimental Service
-
Streptavidin
-
Fast blue Protein Stain solution
-
Single-component Reagents of FLIA Kit
-
Streptavidin-Agarose Beads
Magazine | Citations |
Diabetologia | Translationally controlled tumour protein is associated with podocyte hypertrophy in a mouse model of type 1 diabetes SpringerLink: e14361700w260x75 |
Int Arch Allergy Immunol. | Synergistic Actions of Histamine-Releasing Factor and Histamine Releasing Factor-Reactive IgE in Chronic Urticaria. pubmed:28219065 |
Biomed Pharmacother | dTBP2 attenuates severe airway inflammation by blocking inflammatory cellular network mediated by dTCTP 34628164 |