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CLIA Kit for Integrin Beta 6 (ITGb6)
ITG-B6
- Product No.SCC099Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample Typetissue homogenates, cell lysates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length2h, 40min
- Detection Range13.7-10,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 4.9pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 588
For more details, please contact local distributors! US$ 840 US$ 3780 US$ 7140 US$ 58800
Specificity
This assay has high sensitivity and excellent specificity for detection of Integrin Beta 6 (ITGb6).
No significant cross-reactivity or interference between Integrin Beta 6 (ITGb6) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Integrin Beta 6 (ITGb6) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Integrin Beta 6 (ITGb6) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
Substrate A | 1×10mL | Substrate B | 1×2mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.
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Magazine | Citations |
Intergrin alpha v beta 6 for diagnosis/prognosis of colorectal carcinoma : | |
International Journal of Cancer | β‐Integrin serves as a novel serum tumor marker for colorectal carcinoma Pubmed: 30653264 |
Clin Transl Gastroenterol | β6-Integrin Serves as a Potential Serum Marker for Diagnosis and Prognosis of Pancreatic Adenocarcinoma 34388137 |
Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
RPC099Hu01 | Recombinant Integrin Beta 6 (ITGb6) | Positive Control; Immunogen; SDS-PAGE; WB. |
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MAC099Hu22 | Monoclonal Antibody to Integrin Beta 6 (ITGb6) | WB |
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SEC099Hu | ELISA Kit for Integrin Beta 6 (ITGb6) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCC099Hu | CLIA Kit for Integrin Beta 6 (ITGb6) | Chemiluminescent immunoassay for Antigen Detection. |
LMC099Hu | Multiplex Assay Kit for Integrin Beta 6 (ITGb6) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |