≡

CLIA Kit for Kallikrein 7 (KLK7)

PRSS6; SCCE; hK7; Kallikrein-Related Peptidase 7; Serine protease 6; Stratum corneum chymotryptic enzyme

Specificity

This assay has high sensitivity and excellent specificity for detection of Kallikrein 7 (KLK7).
No significant cross-reactivity or interference between Kallikrein 7 (KLK7) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Kallikrein 7 (KLK7) and the recovery rates were calculated by comparing the measured value to the expected amount of Kallikrein 7 (KLK7) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 82-93 90
EDTA plasma(n=5) 89-103 95
heparin plasma(n=5) 96-105 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kallikrein 7 (KLK7) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kallikrein 7 (KLK7) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Kallikrein 7 (KLK7) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 94-102% 94-102% 79-93% 85-99%
EDTA plasma(n=5) 96-103% 98-105% 93-105% 92-102%
heparin plasma(n=5) 79-101% 79-92% 85-105% 93-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Cellular and Molecular Life Sciences Vaspin inhibits kallikrein 7 by serpin mechanism PubMed: PMC3689916
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB910Hu01 Recombinant Kallikrein 7 (KLK7) Positive Control; Immunogen; SDS-PAGE; WB.
RPB910Hu02 Recombinant Kallikrein 7 (KLK7) Positive Control; Immunogen; SDS-PAGE; WB.
PAB910Hu02 Polyclonal Antibody to Kallikrein 7 (KLK7) WB; IHC; ICC; IP.
PAB910Hu01 Polyclonal Antibody to Kallikrein 7 (KLK7) WB; IHC; ICC; IP.
LAB910Hu81 FITC-Linked Polyclonal Antibody to Kallikrein 7 (KLK7) WB; IHC; ICC; IF.
LAB910Hu71 Biotin-Linked Polyclonal Antibody to Kallikrein 7 (KLK7) WB; IHC; ICC.
MAB910Hu22 Monoclonal Antibody to Kallikrein 7 (KLK7) WB; IHC; ICC; IP.
SEB910Hu ELISA Kit for Kallikrein 7 (KLK7) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB910Hu CLIA Kit for Kallikrein 7 (KLK7) Chemiluminescent immunoassay for Antigen Detection.
LMB910Hu Multiplex Assay Kit for Kallikrein 7 (KLK7) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.