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Figure. Western Blot; Sample: Recombinant ALP, Bovine.
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Polyclonal Antibody to Alkaline Phosphatase (ALP)
AKP; ALKP; Basic Phosphatase
- Product No.PAB472Bo01
- Organism SpeciesBos taurus; Bovine (Cattle) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPB472Bo01-Recombinant Alkaline Phosphatase (ALP)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species Moren/a
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - DownloadInstruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 73
US$ 169
US$ 242
US$ 605
US$ 2420
For more details, please contact local distributors!
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against ALP. It has been selected for its ability to recognize ALP in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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INCREMENT SERVICES
| Magazine | Citations |
| Clinical Oral Implants Research | Comparison of platelet rich fibrin and collagen as osteoblast-seeded scaffolds for bone tissue engineering applications Wiley: source |
| Die Pharmazie - An International Journal of Pharmaceutical Sciences | The role of atorvastatin in bone metabolism in male albino Wistar rats Ingenta: art00010 |
| 22 | Combined effect of strontium and zoledronate on hydroxyapatite structure and bone cell responses ScienceDirect: S0142961214003135 |
| Biomed Mater.? | Directing chondrogenic differentiation of mesenchymal stem cells with a solid-supported chitosan thermogel for cartilage tissue engineering Pubmed:24770944 |
| Journal of Colloid and Interface Science | Strontium and zoledronate hydroxyapatites graded composite coatings for bone prostheses Pubmed:25706198 |
| Bone | The active role of osteoporosis in the interaction between osteoblasts and bone metastases PubMed: 26057367 |
| Macromolecular Bioscience | Multi‐Layered Scaffolds for Osteochondral Tissue Engineering: In Vitro Response of Co‐Cultured Human Mesenchymal Stem Cells PubMed: 26126665 |
| journal of hard Tissue Biology | Biological Evaluation of a Prototype Material made of Polyglycolic Acid and Hydroxyapatite Article: Jhtb |
| Colloids Surf B Biointerfaces | Antiresorption implant coatings based on calcium alendronate and octacalcium phosphate deposited by matrix assisted pulsed laser evaporation PubMed: 26445021 |
| Gels | On the Mechanism of Drug Release from Polysaccharide Hydrogels Cross-Linked with Magnetite Nanoparticles by Applying Alternating Magnetic Fields: the Case of DOXO Delivery 2310-2861: 1 |
| Acta Biomater | Antioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: an in vitro osteoblast-osteoclast-endothelial cell co-culture study PubMed: 26689470 |
| Sci Rep | A composite scaffold of MSC affinity peptide-modified demineralized bone matrix particles and chitosan hydrogel for cartilage regeneration PubMed: 26632447 |
| Acta Biomaterialia | Antioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: An in vitro osteoblast–osteoclast–endothelial cell co-culture study Pubmed:26689470 |
| PLoS One. | Extracorporeal shock waves alone or combined with raloxifene promote bone formation and suppress resorption in ovariectomized rats. pubmed:28158228 |
| Scientific Reports | Adiponectin protects the rats liver against chronic intermittent hypoxia induced injury throughAMP-activated protein kinase pathway. pubmed:27678302 |
| J Cell Physiol. | An advanced tri-culture model to evaluate the dynamic interplay among osteoblasts, osteoclasts, and endothelial cells. pubmed:28240358 |
| Biomedicine & Pharmacotherapy | Anti-osteoporotic effects of an antidepressant tianeptine on ovariectomized rats S0753332216322752 |
| Journal of Inorganic Biochemistry | Biomimetic fabrication of antibacterial calcium phosphates mediated by polydopamine. pubmed:29049953 |
| Biomedicine & pharmacotherapy | Icaritin induces MC3T3-E1 subclone14 cell differentiation through estrogen receptor-mediated ERK1/2 and p38 signaling activation. pubmed:28742995 |
| Journal of Clinical Periodontology | Peroxisome proliferator‐activated receptor γ plays dual roles on experimental periodontitis in rats Pubmed:29574908 |
| Macromolecular Bioscience | Strontium‐Substituted Hydroxyapatite‐Gelatin Biomimetic Scaffolds Modulate Bone Cell Response Pubmed:29877029 |
| Journal of Inorganic Biochemistry | Gradient coatings of strontium hydroxyapatite/zinc β-tricalcium phosphate as a tool to modulate osteoblast/osteoclast response Pubmed:29525694 |
| DNA and Cell Biology | miRNA-133a-5p Inhibits the Expression of Osteoblast Differentiation-Associated Markers by Targeting the 3′ UTR of Pubmed:29359964 |
| Bone | The sole and combined effect of simvastatin and platelet rich fibrin as a filling material in induced bone defect in tibia of albino rats Pubmed: 30208342 |
| Experimental Gerontology | The decline of whole-body glucose metabolism in ovariectomized rats Pubmed: 30292771 |
| BMC Complementary and Alternative Medicine | Hepatoprotective activity of Erythrina× neillii leaf extract and characterization of its phytoconstituents Pubmed: 28095910 |
| Journal of Hematology & Oncology | Multiple myeloma-derived exosomes are enriched of amphiregulin (AREG) and activate the epidermal growth factor pathway in the bone microenvironment … Pubmed: 30621731 |
| JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS | CoCr porous scaffolds manufactured via selective laser melting in orthopedics: Topographical, mechanical, and biological characterization Pubmed: 30689288 |
| Chemico-Biological Interactions | Lead exposure inhibits osteoblastic differentiation and inactivates the canonical Wnt signal and recovery by icaritin in MC3T3-E1 subclone 14 cells Pubmed: 30731080 |
| Journal of Orthopaedic Surgery and Research | Macrophage inhibits the osteogenesis of fibroblasts in ultrahigh molecular weight polyethylene (UHMWPE) wear particle-induced osteolysis |
| Bangladesh Journal of Medical Science | Zn as the Factor of Alkaline Phosphatase in Periodontal Patients consuming Minangkabau food in West Sumatra, Indonesia |
| Molecular Therapy-Nucleic Acids | AAV-anti-miR-214 prevents collapse of the femoral head in osteonecrosis by regulating osteoblast and osteoclast activities Pubmed: 31739209 |
| Journal of Korean Neurosurgical Society | Rutin Improves Bone Histomorphometric Values by Reduction of Osteoclastic Activity in Osteoporosis Mouse Model Induced by Bilateral Ovariectomy Pubmed: 32172552 |
| Journal of Applied Biomaterials & Functional Materials | Effects of tricalcium silicate/sodium alginate/calcium sulfate hemihydrate composite cements on osteogenic performances in vitro and in vivo Pubmed: 32138579 |
| Nutrients | Osteoprotective Activity and Metabolite Fingerprint via UPLC/MS and GC/MS of Lepidium sativum in Ovariectomized Rats Pubmed: 32668691 |
| International Journal of Clinical and Experimental Pathology | Matrilin-2 is a novel prognostic marker in osteosarcoma |
| Revista Argentina de Clínica Psicológica | Research on the Mechanism of miR-142-5p's Promotion of the Function and Mineralization of Osteoblasts by Down-regulating the Expression of WWP1 Gene |
| МАРКЕРЫ КОСТНОГО РЕМОДЕЛИРОВАНИЯ ПРИ ЗАМЕЩЕНИИ ДЕФЕКТАТРАБЕКУЛЯРНОЙ КОСТНОЙ ТКАНИ РЕЗОРБИРУЕМЫМИ И … | |
| Ceramics International | Platinum nanoparticles supported on functionalized hydroxyapatite: Anti-oxidant properties and bone cells response |
| Bioelectromagnetics | Static Magnetic Field (2¨C4 T) Improves Bone Microstructure and Mechanical Properties by Coordinating Osteoblast/Osteoclast Differentiation in Mice 33655538 |
| PeerJ | From hair to liver: emerging application of hair follicle mesenchymal stem cell transplantation reverses liver cirrhosis by blocking the TGF-β/Smad signaling … Pubmed:35186473 |
| Cell Death Discov | ECM1 modified HF-MSCs targeting HSC attenuate liver cirrhosis by inhibiting the TGF-β/Smad signaling pathway Pubmed:35136027 |
| Int J Mol Sci | Antiosteoporotic Nanohydroxyapatite Zoledronate Scaffold Seeded with Bone Marrow Mesenchymal Stromal Cells for Bone Regeneration: A 3D In Vitro Model Pubmed:35682677 |
| Journal of Bionic Engineering | Effect of Antibacterial Enoxacin on the Properties of Injectable Nano-hydroxyapatite/Polyurethane Cement for Bone Repairing |
| Catalog No. | Related products for research use of Bos taurus; Bovine (Cattle) Organism species | Applications (RESEARCH USE ONLY!) |
| RPB472Bo01 | Recombinant Alkaline Phosphatase (ALP) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB472Bo01 | Polyclonal Antibody to Alkaline Phosphatase (ALP) | WB; IHC; ICC; IP. |
| LAB472Bo71 | Biotin-Linked Polyclonal Antibody to Alkaline Phosphatase (ALP) | WB; IHC; ICC. |
| SEB472Bo | ELISA Kit for Alkaline Phosphatase (ALP) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCB472Bo | CLIA Kit for Alkaline Phosphatase (ALP) | Chemiluminescent immunoassay for Antigen Detection. |
| LMB472Bo | Multiplex Assay Kit for Alkaline Phosphatase (ALP) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
| KSB472Bo01 | ELISA Kit DIY Materials for Alkaline Phosphatase (ALP) | Main materials for "Do It (ELISA Kit) Yourself". |