Packages (Simulation)
Image (I)
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DAB staining on IHC-P; Samples: Rat Intestine Tissue; Primary Ab: 20µg/ml Rabbit Anti-Rat H3 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Rat Cerebrum Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Rat H3 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Rat Lung Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Rat H3 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Rat Testis Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Rat H3 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Rat Ovary Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Rat H3 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Cerebrum Tissue
Primary Ab: 10µg/ml Rabbit Anti-Rat H3 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Testis Tissue
Primary Ab: 10µg/ml Rabbit Anti-Rat H3 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Lung Tissue
Primary Ab: 10µg/ml Rabbit Anti-Rat H3 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Image (II)
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Western Blot; Sample: Human Hela cell lysate; Primary Ab: 2µg/ml Rabbit Anti-Rat H3 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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Western Blot; Sample: Human Liver lysate;
Primary Ab: 3µg/ml Rabbit Anti-Rat H3 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human Lung lysate;
Primary Ab: 3µg/ml Rabbit Anti-Rat H3 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human Cartilage lysate;
Primary Ab: 3µg/ml Rabbit Anti-Rat H3 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Rat Bone marrow lysate;
Primary Ab: 2µg/ml Rabbit Anti-Rat H3 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Quality Guarantee
Instruction Manual
Certificate
Polyclonal Antibody to Histone H3 (H3)
- Product No.PAA285Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA285Ra01-Recombinant Histone H3 (H3)
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration0.5mg/ml
- Organism Species MoreHomo sapiens (Human)
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - DownloadInstruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 110
US$ 258
US$ 368
US$ 920
US$ 3680
For more details, please contact local distributors!
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against H3. It has been selected for its ability to recognize H3 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.003-1µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Magazine | Citations |
| Biomed Res Int | A Novel Anti-Histone H1 Monoclonal Antibody, SSV Monoclonal Antibody, Improves Lung Injury and Survival in a Mouse Model of Lipopolysaccharide-Induced Sepsis-Like Syndrome PubMed: 25649890 |
| Cell Death & Disease | Proteomic analysis reveals USP7 as a novel regulator of palmitic acid-induced hepatocellular carcinoma cell death Pubmed:35732625 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA285Ra01 | Recombinant Histone H3 (H3) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA285Ra01 | Polyclonal Antibody to Histone H3 (H3) | WB; IHC; ICC; IP. |
| KSA285Ra01 | ELISA Kit DIY Materials for Histone H3 (H3) | Main materials for "Do It (ELISA Kit) Yourself". |