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OneStep ELISA Kit for Alpha-1-Antitrypsin (a1AT)
SERPINA1; SPAAT; A1-AT; PI; Serpin Peptidase Inhibitor,Clade A(Alpha-1 Antiproteinase/AntiTrypsin)Member 1; Alpha-1 protease inhibitor; Short peptide from AAT; Serpin A1
- Product No.OEB697Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length1h10min
- Detection Range0.25-16ug/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.09ug/mL.
- DownloadInstruction Manual
- UOM 48T96T
- FOB
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Specificity
This assay has high sensitivity and excellent specificity for detection of Alpha-1-Antitrypsin (a1AT).
No significant cross-reactivity or interference between Alpha-1-Antitrypsin (a1AT) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Alpha-1-Antitrypsin (a1AT) and the recovery rates were calculated by comparing the measured value to the expected amount of Alpha-1-Antitrypsin (a1AT) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 95-103 | 99 |
| EDTA plasma(n=5) | 85-101 | 91 |
| heparin plasma(n=5) | 88-97 | 92 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-1-Antitrypsin (a1AT) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-1-Antitrypsin (a1AT) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Alpha-1-Antitrypsin (a1AT) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 79-104% | 85-102% | 92-101% | 85-95% |
| EDTA plasma(n=5) | 80-103% | 81-98% | 92-99% | 94-102% |
| heparin plasma(n=5) | 80-99% | 87-103% | 88-102% | 79-103% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well, then add 50µL prepared Detection Reagent A to each well. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
5. Add 50µL Stop Solution. Read at 450nm immediately.
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| Magazine | Citations |
| Journal of Biotechnology | Expression and characterization of recombinant human alpha-antitrypsin in transgenic rice seed PubMed: 23376844 |
| Sci Rep | Hepatic steatosis depresses alpha-1-antitrypsin levels in human and rat acute pancreatitis PubMed: 26634430 |
| PLoS One | Gut Microbial Dysbiosis May Predict Diarrhea and Fatigue in Patients Undergoing Pelvic Cancer Radiotherapy: A Pilot Study PubMed: 25955845 |
| Respir Care | Diagnostic Utility of Biomarkers in COPD PubMed: 26106205 |
| Acta Anaesthesiologica Scandinavica | Early prognostic factors in septic shock cancer patients: a prospective study with a proteomic approach Pubmed:29315472 |
| Journal of Clinical Medicine | Urinary Proteomics for the Early Diagnosis of Diabetic Nephropathy in Taiwanese Patients Pubmed: 30486327 |
| Journal of Proteomics | Salivary proteins from dysplastic leukoplakia and oral squamous cell carcinoma and their potential for early detection Pubmed: 31706945 |
| Diagnostics | Systemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2J Pubmed: 32585848 |
| Pediatrics International | Prenatal diagnosis of maternal serum from mothers carrying β‐thalassemic fetus 34559910 |