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OneStep ELISA Kit for Coagulation Factor II (F2)
FII; TM; PT; Thrombin; Prothrombin; Pro-Thrombin; Activation peptide fragment 1; Activation peptide fragment 2
- Product No.OEA820Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample Typeserum, plasma and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length1h10min
- Detection Range78-5,000ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 26ng/mL.
- DownloadInstruction Manual
- UOM 48T96T
- FOB
US$
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Specificity
This assay has high sensitivity and excellent specificity for detection of Coagulation Factor II (F2).
No significant cross-reactivity or interference between Coagulation Factor II (F2) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Coagulation Factor II (F2) and the recovery rates were calculated by comparing the measured value to the expected amount of Coagulation Factor II (F2) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 80-88 | 83 |
| EDTA plasma(n=5) | 85-103 | 95 |
| heparin plasma(n=5) | 78-101 | 81 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Coagulation Factor II (F2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Coagulation Factor II (F2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Coagulation Factor II (F2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-88% | 92-101% | 91-102% | 96-103% |
| EDTA plasma(n=5) | 97-105% | 98-105% | 90-104% | 95-102% |
| heparin plasma(n=5) | 89-96% | 96-104% | 95-103% | 94-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well, then add 50µL prepared Detection Reagent A to each well. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
5. Add 50µL Stop Solution. Read at 450nm immediately.
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| Magazine | Citations |
| Blood Cells Mol Dis | Upregulation of cytokine signalling in platelets increases risk of thrombophilia in severe COVID-19 patients Pubmed:35180460 |