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OneStep ELISA Kit for Hexosaminidase B Beta (HEXb)
Hex-B; ENC-1AS; HCC-7; Beta-N-acetylhexosaminidase subunit beta; Cervical cancer proto-oncogene 7 protein; N-acetyl-beta-glucosaminidase subunit beta
- Product No.OEA637Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length1h10min
- Detection Range0.78-50ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.31ng/mL.
- DownloadInstruction Manual
- UOM 48T96T
- FOB
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Specificity
This assay has high sensitivity and excellent specificity for detection of Hexosaminidase B Beta (HEXb).
No significant cross-reactivity or interference between Hexosaminidase B Beta (HEXb) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Hexosaminidase B Beta (HEXb) and the recovery rates were calculated by comparing the measured value to the expected amount of Hexosaminidase B Beta (HEXb) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 88-103 | 92 |
| EDTA plasma(n=5) | 92-99 | 95 |
| heparin plasma(n=5) | 79-97 | 80 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hexosaminidase B Beta (HEXb) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hexosaminidase B Beta (HEXb) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Hexosaminidase B Beta (HEXb) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 91-102% | 86-99% | 81-98% | 96-103% |
| EDTA plasma(n=5) | 89-101% | 96-104% | 78-105% | 98-105% |
| heparin plasma(n=5) | 88-103% | 80-101% | 83-99% | 98-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well, then add 50µL prepared Detection Reagent A to each well. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
5. Add 50µL Stop Solution. Read at 450nm immediately.
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| Magazine | Citations |
| The American Journal of Chinese Medicine | Uncovering the Molecular Mechanism of Anti-Allergic Activity of Silkworm Pupa-Grown Cordyceps militaris Fruit Body. pubmed:28367714 |
| Life Sciences | Resveratrol efficiently improves pulmonary function via stabilizing mast cells in a rat intestinal injury model pubmed:28733146 |
| Neural Regen Res | Functionality of a bicistronic construction containing HEXA and HEXB genes encoding ¦Â-hexosaminidase A for cell-mediated therapy of GM2 gangliosidoses 34100447 |
| Nat Commun | The C5a/C5a receptor 1 axis controls tissue neovascularization through CXCL4 release from platelets 34099640 |
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
| EPA637Mu61 | Eukaryotic Hexosaminidase B Beta (HEXb) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA637Mu01 | Recombinant Hexosaminidase B Beta (HEXb) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA637Mu02 | Recombinant Hexosaminidase B Beta (HEXb) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA637Mu01 | Polyclonal Antibody to Hexosaminidase B Beta (HEXb) | WB; IHC; ICC/IF |
| PAA637Mu02 | Polyclonal Antibody to Hexosaminidase B Beta (HEXb) | IHC; ICC/IF |
| OEA637Mu | OneStep ELISA Kit for Hexosaminidase B Beta (HEXb) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA637Mu | ELISA Kit for Hexosaminidase B Beta (HEXb) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA637Mu | Multiplex Assay Kit for Hexosaminidase B Beta (HEXb) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
| CMA637Mu | Multiplex Assay Kit for Hexosaminidase B Beta (HEXb) ,etc. by CBA (Cytometric Bead Array) | FLIA Kit for Antigen Detection. |