Mini Samples ELISA Kit for Creatine Kinase, Muscle (CKM)

CK-MM; CKMM; CK3; CK-M; MM-CK; M-CK; Creatine kinase M-type, N-terminally processed

Specificity

This assay has high sensitivity and excellent specificity for detection of Mini Samples Creatine Kinase, Muscle (CKM).
No significant cross-reactivity or interference between Mini Samples Creatine Kinase, Muscle (CKM) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Mini Samples Creatine Kinase, Muscle (CKM) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Creatine Kinase, Muscle (CKM) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 87-94 91
EDTA plasma(n=5) 81-96 91
heparin plasma(n=5) 91-101 96

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Creatine Kinase, Muscle (CKM) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Creatine Kinase, Muscle (CKM) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Creatine Kinase, Muscle (CKM) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 89-98% 95-104% 78-105% 96-103%
EDTA plasma(n=5) 78-103% 99-105% 81-92% 92-99%
heparin plasma(n=5) 80-95% 82-91% 87-94% 85-96%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×60µL Assay Diluent A 1×6mL
Detection Reagent B 1×60µL Assay Diluent B 1×6mL
TMB Substrate 1×4.5mL Stop Solution 1×3mL
Wash Buffer (30 × concentrate) 1×10mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Journal of Inflammation Assessment of endothelium and inflammatory response at the onset of reperfusion injury in hand surgery PubMed: 22583529
PLoS One Extraocular Muscle Characteristics Related to Myasthenia Gravis Susceptibility PubMed: PMC3568149
Kidney Int. Fructokinase activity mediates dehydration-induced renal injury Pubmed: 24336030
Methylxanthines and cardiotoxicity Methylxanthine induced cardiotoxicity and its mechanisms: An experimental study 230b:34bf146150b42971d76dda5e8329bc5f2f0b.pdf
Molecular Medicine Reports Protective effects of N (2)‑L‑alanyl‑L‑glutamine mediated by the JAK2/STAT3 signaling pathway on myocardial ischemia reperfusion Pubmed:29393473
Toxicological Sciences Proteomic and Metabolomic Profiling Identify Plasma Biomarkers for Exposure to Ultra-low Levels of Carfentanil Pubmed: 30321427
Clinical & Translational Oncology The effects of human sera conditioned by high-intensity exercise sessions and training on the tumorigenic potential of cancer cells Pubmed: 32447643
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA109Mu01 Recombinant Creatine Kinase, Muscle (CKM) Positive Control; Immunogen; SDS-PAGE; WB.
RPA109Mu02 Recombinant Creatine Kinase, Muscle (CKM) Positive Control; Immunogen; SDS-PAGE; WB.
PAA109Mu01 Polyclonal Antibody to Creatine Kinase, Muscle (CKM) WB; IHC; ICC; IP.
PAA109Mu02 Polyclonal Antibody to Creatine Kinase, Muscle (CKM) WB; IHC
LAA109Mu71 Biotin-Linked Polyclonal Antibody to Creatine Kinase, Muscle (CKM) WB; IHC; ICC.
LAA109Mu81 FITC-Linked Polyclonal Antibody to Creatine Kinase, Muscle (CKM) WB; IHC; ICC; IF.
MAA109Mu21 Monoclonal Antibody to Creatine Kinase, Muscle (CKM) IHC
LAA109Mu72 Biotin-Linked Monoclonal Antibody to Creatine Kinase, Muscle (CKM) WB; IHC; ICC.
MEA109Mu Mini Samples ELISA Kit for Creatine Kinase, Muscle (CKM) Enzyme-linked immunosorbent assay for Antigen Detection.
SEA109Mu ELISA Kit for Creatine Kinase, Muscle (CKM) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA109Mu Multiplex Assay Kit for Creatine Kinase, Muscle (CKM) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
PSA109Mu01 Antibody Pair for Creatine Kinase, Muscle (CKM) ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA109Mu01 ELISA Kit DIY Materials for Creatine Kinase, Muscle (CKM) Main materials for "Do It(ELISA Kit) Yourself"