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Mini Samples ELISA Kit for Interleukin 18 (IL18)
IGIF; IL-1g; IL1F4; IL1-F4; Interferon-Gamma-Inducing Factor; Interleukin-1 Family Member 4; Iboctadekin; Interleukin-1 Gamma
- Product No.MEA064Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range15.6-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 5.5pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 470
US$ 672
US$ 3024
US$ 5712
US$ 47040
For more details, please contact local distributors!
Specificity
This assay has high sensitivity and excellent specificity for detection of Mini Samples Interleukin 18 (IL18).
No significant cross-reactivity or interference between Mini Samples Interleukin 18 (IL18) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Mini Samples Interleukin 18 (IL18) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Interleukin 18 (IL18) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 83-94 | 88 |
| EDTA plasma(n=5) | 95-104 | 101 |
| heparin plasma(n=5) | 83-90 | 87 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Interleukin 18 (IL18) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Interleukin 18 (IL18) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Interleukin 18 (IL18) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-95% | 84-94% | 82-94% | 90-97% |
| EDTA plasma(n=5) | 98-105% | 93-105% | 86-102% | 89-96% |
| heparin plasma(n=5) | 91-105% | 95-102% | 81-101% | 81-89% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×60µL | Assay Diluent A | 1×6mL |
| Detection Reagent B | 1×60µL | Assay Diluent B | 1×6mL |
| TMB Substrate | 1×4.5mL | Stop Solution | 1×3mL |
| Wash Buffer (30 × concentrate) | 1×10mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.
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| Magazine | Citations |
| International Immunopharmacology | Curcumin immune-mediated and anti-apoptotic mechanisms protect against renal ischemia/reperfusion and distant organ induced injuries ScienceDirect: S1567576911001068 |
| Atherosclerosis | IL-18 overexpression promotes vascular inflammation and remodeling in a rat model of metabolic syndrome ScienceDirect: S0021915009006200 |
| PLoS One. | HIF-1α Is Essential for Effective PMN Bacterial Killing, Antimicrobial Peptide Production and Apoptosis in Pseudomonas aeruginosa Keratitis Plos: Source |
| 临床儿科杂志 | 中性粒细胞明胶酶相关脂质运载蛋白、肾损伤分子1和白介素18 在诊断心肺分流术后急性肾损伤中的意义 Jcp-Sh:Source |
| Cell Death Dis. | MicroRNA-30d regulates cardiomyocyte pyroptosis by directly targeting foxo3a in diabetic cardiomyopathy Pubmed:Pmc4237254 |
| PLoS One. | Cilostazol Renoprotective Effect: Modulation of PPAR-γ, NGAL, KIM-1 and IL-18 Underlies Its Novel Effect in a Model of Ischemia-Reperfusion Pubmed:24816434 |
| J Korean Med Sci. | Acute Kidney Injury after Using Contrast during Cardiac Catheterization in Children with Heart Disease Pubmed:25120320 |
| PLOS ONE | Protective Effect of Leaf Essential Oil from PubMed: 25794175 |
| Fertil Steril | Inflammosome in the human endometrium: further step in the evaluation of the “maternal side” PubMed: 26474737 |
| United States Patent Application | GLUCAN-ENCAPSULATED SIRNA FOR TREATING TYPE 2 DIABETES MELLITUS y2016:0138028.html |
| Front Pharmacol | Poria Attenuates Idiosyncratic Liver Injury Induced by Polygoni Multiflori Radix Praeparata pubmed:27803670 |
| Pharmacological reports | The Role of PON-1, GR, IL-18, and oxLDL in Depression With and Without Posttraumatic Stress Disorder science:article |
| International Journal of Molecular Sciences | PEDF Inhibits the Activation of NLRP3 Inflammasome in Hypoxia Cardiomyocytes through PEDF Receptor/Phospholipase A2 pubmed:27973457 |
| Cellular Physiology and Biochemistry | MiR-155 is Involved in Renal Ischemia-Reperfusion Injury via Direct Targeting of FoxO3a andRegulating Renal Tubular Cell Pyroptosis. pubmed:28006785 |
| Journal of Food and Drug Analysis | Anti-inflammatory effect of cinnamaldehyde and linalool from the leaf essential oil of Cinnamomum osmophloeum Kanehira in endotoxin-induced … S1021-9498(17)30081-9 |
| Journal of Microbiology, Immunology and Infection | Identification of a panel of serum protein markers in early stage of sepsis and its validation in a cohort of patients pubmed:28655573 |
| Discovery and Validation of Immunological Biomarkers in Milk for Health Monitoring of Dairy Cows - Results from a Multiomics Approach doi:10.4172/2329-888X.1000182 | |
| Cellular Physiology and Biochemistry | Dexmedetomidine Alleviates HyperoxiaInduced Acute Lung Injury via Inhibiting NLRP3 Inflammasome Activation pubmed:28873369 |
| 20 | Melatonin prevents endothelial cell pyroptosis via regulation of long noncoding RNA MEG3/miR-223/NLRP3 axis pubmed:29024030 |
| Pharmacological Reports | The role of PON-1, GR, IL-18, and OxLDL in depression with and without posttraumatic stress disorder. pubmed:28623707 |
| World journal of gastroenterology | Hypothermic machine perfusion with metformin-University of Wisconsin solution for ex vivo preservation of standard and marginal liver grafts in a rat model. pubmed:29142469 |
| Veterinary microbiology | Secondary Haemophilus parasuis infection enhances highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) infection-mediated inflammatory responses. pubmed:28532803 |
| International immunopharmacology | Chronic dexamethasone treatment results in hippocampal neurons injury due to activate NLRP1 inflammasome in vitro pubmed:28605710 |
| Journal of Neuroinflammation | Chronic glucocorticoid exposure activates BK-NLRP1 signal involving in hippocampal neuron damage. pubmed:28732502 |
| Journal of Neuroinflammation | Unconjugated bilirubin induces pyroptosis in cultured rat cortical astrocytes Pubmed:29357878 |
| Oxidative Medicine and Cellular Longevity | The Effects of Sinapic Acid on the Development of Metabolic Disorders Induced by Estrogen Deficiency in Rats Pubmed:29967666 |
| Journal of Translational Medicine | Recurrent pregnancy loss is associated to leaky gut: a novel pathogenic model of endometrium inflammation? Pubmed:29665864 |
| Chemico-Biological Interactions | Nifuroxazide, a STAT3 inhibitor, mitigates inflammatory burden and protects against diabetes-induced nephropathy in rats Pubmed:29291386 |
| Journal of Food and Drug Analysis | Anti-inflammatory effect of cinnamaldehyde and linalool from the leaf essential oil of Cinnamomum osmophloeum Kanehira in endotoxin-induced mice Pubmed:29389558 |
| International Journal of Molecular Medicine | MCMV triggers ROS/NLRP3-associated inflammasome activation in the inner ear of mice and cultured spiral ganglion neurons, contributing to sensorineural hearing … Pubmed:29512778 |
| Archives of Virology | Genetic and immunogenicity analysis of porcine circovirus type 2 strains isolated in central China Pubmed:29305646 |
| Journal of Inflammation | Expression and clinical significance of the NEK7-NLRP3 inflammasome signaling pathway in patients with systemic lupus erythematosus Pubmed: 30202244 |
| Journal of Affective Disorders | Post-stroke depression and cognitive impairment: study design and preliminary findings in a Brazilian prospective stroke cohort (EMMA study) Doi: 10.1016/j.jad.2018.10.003 |
| BioScientia Medicina | Sensitivity and Spesificity of Interleukin 18 as Biomarker for Acute Kidney Injury |
| International Journal of Molecular Medicine | Ginsenoside Rg1 protects against H2O2‑induced neuronal damage due to inhibition of the NLRP1 inflammasome signalling pathway in hippocampal neurons in vitro Pubmed: 30535505 |
| Nutrients | Effect of Rosmarinic Acid on the Serum Parameters of Glucose and Lipid Metabolism and Oxidative Stress in Estrogen-Deficient Rats Pubmed: 30691017 |
| International immunopharmacology | NADPH oxidase 2-mediated NLRP1 inflammasome activation involves in neuronal senescence in hippocampal neurons in vitro Pubmed: 30677569 |
| Acta Histochemica | Pre-administration of luteoline attenuates neonatal sevoflurane-induced neurotoxicity in mice Doi: 10.1016/j.acthis.2019.04.004 |
| JOURNAL OF DRUG TARGETING | Artemisinin alleviates atherosclerotic lesion by reducing macrophage inflammation via regulation of AMPK/NF-κB/NLRP3 inflammasomes pathway Pubmed: 31094238 |
| IUBMB Life | Melatonin Enhances Autophagy and Decreases Apoptosis Induced by nanosilica in RAW264. 7 cells Pubmed: 31018046 |
| American Journal of Reproductive Immunology | Effect of alpha lipoic acid and myoinositol on endometrial inflammasome from recurrent pregnancy loss women Pubmed: 31148259 |
| INFLAMMATION RESEARCH | TNF-α/calreticulin dual signaling induced NLRP3 inflammasome activation associated with HuR nucleocytoplasmic shuttling in rheumatoid arthritis Pubmed: 31119302 |
| PLoS One | Sepsis causes right ventricular myocardial inflammation independent of pulmonary hypertension in a porcine sepsis model Pubmed: 31247004 |
| Journal of Dairy Science | Hepatic nuclear factor kappa B signaling pathway and NLR family pyrin domain containing 3 inflammasome is over-activated in ketotic dairy cows Pubmed: 31495623 |
| Rheumatology international | Serum levels of selected cytokines [interleukin (IL)-17A, IL-18, IL-23] and chemokines (RANTES, IP10) in the acute phase of immunoglobulin A vasculitis in … Pubmed: 31468124 |
| LIFE SCIENCES | Protective effects of epigallocatechin gallate against ischemia reperfusion injury in rat skeletal muscle via activating Nrf2/HO-1 signaling pathway Pubmed: 31678278 |
| life sciences | Melatonin biosynthesis restored by CpG oligodeoxynucleotides attenuates allergic airway inflammation via regulating NLRP3 inflammasome Pubmed: 31738882 |
| International Immunopharmacology | Dexamethasone alleviate allergic airway inflammation in mice by inhibiting the activation of NLRP3 inflammasome Pubmed: 31780368 |
| Journal of Food Science | Effects of Total Flavonoids from Exocarpium Citri Grandis on Air Pollution Particle‐Induced Pulmonary Inflammation and Oxidative Stress in Mice Pubmed: 31762039 |
| Antioxidants | Effect of Rosmarinic Acid and Sinapic Acid on Oxidative Stress Parameters in the Cardiac Tissue and Serum of Type 2 Diabetic Female Rats Pubmed: 31771099 |
| Frontiers in Immunology | The Increase in IL-1β in the Early Stage of Heatstroke Might Be Caused by Splenic Lymphocyte Pyroptosis Induced by mtROS-Mediated Activation of the … Pubmed: 31921131 |
| Pharmacological Reports | The dual reno-and neuro-protective effects of dimethyl fumarate against uremic encephalopathy in a renal ischemia/reperfusion model Pubmed: 32141026 |
| Experimental and Therapeutic Medicine | Induction of autophagy protects human dental pulp cells from lipopolysaccharide‑induced pyroptotic cell death Pubmed: 32104285 |
| Cancer Chemother Pharmacol | Novel complementary antitumour effects of celastrol and metformin by targeting IκBκB, apoptosis and NLRP3 inflammasome activation in diethylnitrosamine-induced … Pubmed: 31989218 |
| Frontiers in Immunology | TLR2-Melatonin Feedback Loop Regulates the Activation of NLRP3 Inflammasome in Murine Allergic Airway Inflammation Pubmed: 32117301 |
| molecular immunology | STAT5a induces endotoxin tolerance by alleviating pyroptosis in kupffer cells Pubmed: 32298872 |
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| molecules | Juglone Suppresses LPS-induced Inflammatory Responses and NLRP3 Activation in Macrophages Pubmed: 32646056 |
| molecular immunology | TLR9 mediates the activation of NLRP3 inflammasome and oxidative stress in murine allergic airway inflammation Pubmed: 32623292 |
| Inflammopharmacology | Novel complementary coloprotective effects of metformin and MCC950 by modulating HSP90/NLRP3 interaction and inducing autophagy in rats Pubmed: 32594364 |
| Life Sci | Puerarin ameliorates retinal ganglion cell damage induced by retinal ischemia/reperfusion through inhibiting the activation of TLR4/NLRP3 inflammasome Pubmed: 32526286 |
| Toxicol Appl Pharmacol | Celastrol augments sensitivity of NLRP3 to CP-456773 by modulating HSP-90 and inducing autophagy in dextran sodium sulphate-induced colitis in rats Pubmed: 32470352 |
| STEM CELL REVIEWS AND REPORTS | A Novel Evidence That Mannan Binding Lectin (MBL) Pathway of Complement Cascade Activation is Involved in Homing and Engraftment of Hematopoietic Stem … Pubmed: 32406006 |
| Biomedicine & Pharmacotherapy | Cepharanthine attenuates cerebral ischemia/reperfusion injury by reducing NLRP3 inflammasome-induced inflammation and oxidative stress via inhibiting 12 … Pubmed: 32559840 |
| Molecular Medicine Reports | Effects of PYRIN-containing Apaf1-like protein 1 on isoflurane-induced postoperative cognitive dysfunction in aged rats Pubmed: 32626997 |
| Food and Agricultural Immunology | Acrylamide induced the activation of NLRP3 inflammasome via ROS-MAPKs pathways in Kupffer cells |
| FASEB JOURNAL | Lactobacillus johnsonii L531 ameliorates enteritis via elimination of damaged mitochondria and suppression of SQSTM1‐dependent mitophagy in a Salmonella … |
| Iranian Journal of Allergy, Asthma and Immunology | Increased Level of Caspase-1 in the Serum of Relapsing-remitting Multiple Sclerosis (RRMS) Patients |
| Biomarkers of Allergic Asthma and their Association with Serum Parameters | |
| Drug Dev Res | Paeoniflorin inhibited nod©\like receptor protein©\3 inflammasome and NF©\¦ÊB©\mediated inflammatory reactions in diabetic foot ulcer by inhibiting the chemokine?¡ 33236457 |
| Molecules | Alpha-Lipoic Acid Plays a Role in Endometriosis: New Evidence on Inflammasome-Mediated Interleukin Production, Cellular Adhesion and Invasion 33430011 |
| Mol Cell Biochem | Intermittent high glucose induces pyroptosis of rat H9C2 cardiomyocytes via sodium¨Cglucose cotransporter 1 33608832 |
| Life Sci | Inhibition of SGLT1 protects against glycemic variability-induced cardiac damage and pyroptosis of cardiomyocytes in diabetic mice 33508297 |
| Tissue Cell | Protective effects of Oridonin against cerebral ischemia/reperfusion injury by inhibiting the NLRP3 inflammasome activation 33676236 |
| Inflammopharmacology | Dapagliflozin, an SGLT2 inhibitor, ameliorates acetic acid-induced colitis in rats by targeting NF¦ÊB/AMPK/NLRP3 axis 34002329 |
| Arch Biochem Biophys | Vitamin D ameliorates high-fat-diet-induced hepatic injury via inhibiting pyroptosis and alters gut microbiota in rats 33965368 |
| Placenta | Vitamin D alleviates hypoxia/reoxygenation-induced injury of human trophoblast HTR-8 cells by activating autophagy 34126416 |
| Mol Med Rep | ¦Ê?opioid receptor agonist, U50488H, inhibits pyroptosis through NLRP3 via the Ca2+/CaMKII/CREB signaling pathway and improves synaptic plasticity in ¡ 34036389 |
| J Mol Cell Cardiol | CircHelz activates NLRP3 inflammasome to promote myocardial injury by sponging miR-133a-3p in mouse ischemic heart 34043986 |
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| J Neuroinflammation | Inhibition of heat shock protein family A member 8 attenuates spinal cord ischemia–reperfusion injury via astrocyte NF-κB/NLRP3 inflammasome pathway 34362408 |
| Mol Med Rep | Sacubitril/valsartan (LCZ696) reduces myocardial injury following myocardial infarction by inhibiting NLRP3‑induced pyroptosis via the TAK1/JNK signaling pathway 34296299 |
| Int Immunopharmacol | The protective effect of isoflurane pretreatment on liver IRI by suppressing noncanonical pyroptosis of liver macrophages 34332342 |
| LIFE SCIENCES | The NLRP3 inhibitor dapansutrile attenuates folic acid induced nephrotoxicity via inhibiting inflammasome/caspase-1/IL axis and regulating autophagy/proliferation 34560082 |
| Biochim Biophys Acta Mol Cell Biol Lipids | Elaidic acid induced NLRP3 inflammasome activation via ERS-MAPK signaling pathways in Kupffer cells 34610469 |
| Inflammation | Emodin Attenuates LPS-Induced Acute Lung Injury by Inhibiting NLRP3 Inflammasome-Dependent Pyroptosis Signaling Pathway In vitro and In vivo 34787801 |
| J Agric Food Chem | Allicin Ameliorates Intestinal Barrier Damage via Microbiota-Regulated Short-Chain Fatty Acids-TLR4/MyD88/NF-κB Cascade Response in Acrylamide-Induced Rats 34694121 |
| Journal of Inflammation Research | The Supportive Role of NSC328382, a P2X7R Antagonist, in Enhancing the Inhibitory Effect of CRID3 on NLRP3 Inflammasome Activation in Rats with Dextran … 34321905 |
| Viruses | Interleukin-10-Mediated Lymphopenia Caused by Acute Infection with Foot-and-Mouth Disease Virus in Mice 34960627 |
| Toxicology | Salidroside alleviates liver inflammation in furan-induced mice by regulating oxidative stress and endoplasmic reticulum stress 34450210 |
| Antioxidants | Key Mechanisms and Potential Implications of Hericium erinaceus in NLRP3 Inflammasome Activation by Reactive Oxygen Species during Alzheimer's Disease 34829535 |
| Ecotoxicol Environ Saf | Silica nanoparticles induce pulmonary autophagy dysfunction and epithelial-to-mesenchymal transition via p62/NF-κB signaling pathway Pubmed:35158278 |
| J Dairy Sci | Overactivation of hepatic mechanistic target of rapamycin kinase complex 1 (mTORC1) is associated with low transcriptional activity of transcription factor EB … Pubmed:35248377 |
| Free Radic Biol Med | Silica nanoparticles induce pyroptosis and cardiac hypertrophy via ROS/NLRP3/Caspase-1 pathway Pubmed:35219847 |
| International Heart Journal | Melatonin Inhibits OGD/R-Induced H9c2 Cardiomyocyte Pyroptosis via Regulation of MT2/miR-155/FOXO3a/ARC Axis Pubmed:35354753 |
| Journal of Integrative Neuroscience | Gastrodin suppresses pyroptosis and exerts neuroprotective effect in traumatic brain injury model by inhibiting NLRP3 inflammasome signaling pathway Pubmed:35364660 |
| Perfusion | Insulin reduces pyroptosis-induced inflammation by PDHA1 dephosphorylation-mediated NLRP3 activation during myocardial ischemia-reperfusion injury Pubmed:35506656 |
| Ecotoxicol Environ Saf | Ambient ozone exposure induces ROS related-mitophagy and pyroptosis via NLRP3 inflammasome activation in rat lung cells Pubmed:35642860 |
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