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Multiplex Assay Kit for Family With Sequence Similarity 3, Member B (FAM3B) ,etc. by FLIA (Flow Luminescence Immunoassay)

ORF9; PANDER; PRED44; C21orf11; C21orf76; Cytokine-like protein 2-21; Pancreatic-derived factor

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Family With Sequence Similarity 3, Member B (FAM3B) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Family With Sequence Similarity 3, Member B (FAM3B) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Family With Sequence Similarity 3, Member B (FAM3B) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Family With Sequence Similarity 3, Member B (FAM3B) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 86-94 90
EDTA plasma(n=5) 78-97 84
heparin plasma(n=5) 82-96 86

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Family With Sequence Similarity 3, Member B (FAM3B) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Family With Sequence Similarity 3, Member B (FAM3B) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Family With Sequence Similarity 3, Member B (FAM3B) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 90-104% 78-89% 89-96% 96-103%
EDTA plasma(n=5) 88-102% 82-95% 89-96% 81-99%
heparin plasma(n=5) 97-105% 92-101% 85-97% 95-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:FAM3B) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
Journal of Diabetes Investigation Elevated circulating level of a cytokine, pancreatic-derived factor, is associated with metabolic syndrome components in a Chinese population Doi: 10.1111
Molecular and Cellular Endocrinology Pancreatic-derived factor impaired glucagon-like Peptide-1 production from GLUTag enterendorine L-cell line and intestines pubmed:28549991
Scientific Reports FAM3B mediates high glucose-induced vascular smooth muscle cell proliferation and migration via inhibition of miR-322-5p pubmed:28536423
Journal of diabetes investigation Effects of circulating FAM3B on the risk of developing metabolic syndrome and its components: a five‐year prospective study pubmed:29178453
Pamukkale Tıp Dergisi The evaluation of serum Pancreatic-derived factor and Malondialdehyde levels in patients with polycystic ovary syndrome
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