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Multiplex Assay Kit for Lysophosphatidic Acid (LPA) ,etc. by FLIA (Flow Luminescence Immunoassay)
LysoPA; 1-Oleoyl-sn-Glycero-3-Phosphate; Lysophosphatidyl Acid
(Note: Up to 8-plex in one testing reaction)
- Product No.LMK623Ge
- Organism SpeciesPan-species (General) Same name, Different species.
- Sample Typeserum, plasma and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length1.5h
- Detection Range9.77-10000ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 3.257 ng/mL.
- DownloadInstruction Manual
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
- FOB
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Result
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Specificity
This assay has high sensitivity and excellent specificity for detection of Lysophosphatidic Acid (LPA) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Lysophosphatidic Acid (LPA) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of Lysophosphatidic Acid (LPA) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Lysophosphatidic Acid (LPA) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 94-104 | 99 |
| EDTA plasma(n=5) | 98-105 | 102 |
| heparin plasma(n=5) | 81-90 | 86 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lysophosphatidic Acid (LPA) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lysophosphatidic Acid (LPA) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Lysophosphatidic Acid (LPA) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-101% | 78-103% | 79-93% | 83-103% |
| EDTA plasma(n=5) | 91-101% | 97-105% | 90-97% | 85-94% |
| heparin plasma(n=5) | 91-99% | 82-97% | 81-104% | 96-104% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:LPA) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
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| Magazine | Citations |
| Molecular Sciences | Pigment Epithelium-Derived Factor (PEDF) Protects Osteoblastic Cell Line from Glucocorticoid-Induced Apoptosis via PEDF-R Pubmed:27187377 |
| Nature Communications | ER-residential Nogo-B accelerates NAFLD-associated HCC mediated by metabolic reprogramming of oxLDL lipophagy Pubmed: 31358770 |
| JOURNAL OF THROMBOSIS AND HAEMOSTASIS | Lysophosphatidic acid promotes thrombus stability by inducing rapid formation of neutrophil extracellular traps: A new mechanism of thrombosis Pubmed: 32291893 |
| Signal Transduction and Targeted Therapy | The Agpat4/LPA axis in colorectal cancer cells regulates antitumor responses via p38/p65 signaling in macrophages Pubmed: 32296017 |
| Journal of Neuroinflammation | MAPK signaling determines lysophosphatidic acid (LPA)-induced inflammation in microglia Pubmed: 32326963 |
| Catalog No. | Related products for research use of Pan-species (General) Organism species | Applications (RESEARCH USE ONLY!) |
| CEK623Ge | ELISA Kit for Lysophosphatidic Acid (LPA) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMK623Ge | Multiplex Assay Kit for Lysophosphatidic Acid (LPA) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |