≡

Multiplex Assay Kit for A Disintegrin And Metalloprotease 28 (ADAM28) ,etc. by FLIA (Flow Luminescence Immunoassay)

ADAM23; MDCL; eMDCII; Metalloproteinase-like, disintegrin-like, and cysteine-rich protein L; Epididymal metalloproteinase-like, disintegrin-like, and cysteine-rich protein II

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of A Disintegrin And Metalloprotease 28 (ADAM28) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between A Disintegrin And Metalloprotease 28 (ADAM28) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant A Disintegrin And Metalloprotease 28 (ADAM28) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of A Disintegrin And Metalloprotease 28 (ADAM28) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 96-104 101
EDTA plasma(n=5) 91-101 96
heparin plasma(n=5) 91-105 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 28 (ADAM28) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 28 (ADAM28) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of A Disintegrin And Metalloprotease 28 (ADAM28) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-105% 99-105% 86-94% 90-102%
EDTA plasma(n=5) 81-104% 94-101% 95-105% 90-98%
heparin plasma(n=5) 97-105% 91-101% 86-101% 92-99%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:ADAM28) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Rapid Communications in Mass Spectrometry Urinary protein profiling by liquid chromatography/tandem mass spectrometry: ADAM28 is overexpressed in bladder transitional cell carcinoma Pubmed: 21913264
Medical Oncology Baseline and decline of serum ADAM28 during chemotherapy of advanced non-small cell lung cancer: a probable predictive and prognostic factor SpringerLink: kg628446760r60rl
Immunology & Cell Biology ADAM28 is elevated in humans with the metabolic syndrome and is a novel sheddase of human tumour necrosis factor-α. PubMed: 23010875
Leuk Res ADAM28 overexpression regulated via the PI3K/Akt pathway is associated with relapse in PubMed: 26340916
Disease Markers Adamalysines as Biomarkers and a Potential Target of Therapy in Colorectal Cancer Patients: Preliminary Results Pubmed: 31565100
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPK202Hu01 Recombinant A Disintegrin And Metalloprotease 28 (ADAM28) Positive Control; Immunogen; SDS-PAGE; WB.
PAK202Hu01 Polyclonal Antibody to A Disintegrin And Metalloprotease 28 (ADAM28) WB; IHC; ICC; IP.
MAK202Hu21 Monoclonal Antibody to A Disintegrin And Metalloprotease 28 (ADAM28) WB; IHC; ICC; IP.
SEK202Hu ELISA Kit for A Disintegrin And Metalloprotease 28 (ADAM28) Enzyme-linked immunosorbent assay for Antigen Detection.
LMK202Hu Multiplex Assay Kit for A Disintegrin And Metalloprotease 28 (ADAM28) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.