Multiplex Assay Kit for Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK) ,etc. by FLIA (Flow Luminescence Immunoassay)

BCKDHKIN; 3-Methyl-2-Oxobutanoate Dehydrogenase/Lipoamide Kinase,Mitochondrial

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:BCKDK) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPJ560Ra01 Recombinant Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK) Positive Control; Immunogen; SDS-PAGE; WB.
PAJ560Ra01 Polyclonal Antibody to Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK) WB; IHC; ICC; IP.
MAJ560Ra21 Monoclonal Antibody to Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK) WB; IHC; ICC; IP.
SEJ560Ra ELISA Kit for Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK) Enzyme-linked immunosorbent assay for Antigen Detection.
LMJ560Ra Multiplex Assay Kit for Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.