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Multiplex Assay Kit for Tetrahydrofolic Acid (THFA) ,etc. by FLIA (Flow Luminescence Immunoassay)
H4FA; THF; Tetrahydrofolate
(Note: Up to 8-plex in one testing reaction)
- Product No.LMG411Ge
- Organism SpeciesPan-species (General) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length1.5h
- Detection Range0.1-100ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.033 ng/mL.
- DownloadInstruction Manual
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
- FOB
US$ 509
US$ 529
US$ 558
US$ 597
US$ 636
US$ 695
US$ 783
Result
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Specificity
This assay has high sensitivity and excellent specificity for detection of Tetrahydrofolic Acid (THFA) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Tetrahydrofolic Acid (THFA) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of Tetrahydrofolic Acid (THFA) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Tetrahydrofolic Acid (THFA) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 81-101 | 92 |
EDTA plasma(n=5) | 91-103 | 95 |
heparin plasma(n=5) | 93-101 | 96 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tetrahydrofolic Acid (THFA) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tetrahydrofolic Acid (THFA) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Tetrahydrofolic Acid (THFA) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 89-103% | 95-105% | 80-101% | 95-102% |
EDTA plasma(n=5) | 78-89% | 96-104% | 80-91% | 97-104% |
heparin plasma(n=5) | 79-99% | 82-92% | 83-93% | 95-102% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
96-well plate | 1 | Plate sealer for 96 wells | 4 |
Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
Pre-Mixed Magnetic beads (22#:THFA) | 1 | Analysis buffer | 1×20mL |
Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
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INCREMENT SERVICES
Magazine | Citations |
Scientifica | Assessment of Folic Acid Supplementation in Pregnant Women by Estimation of Serum Levels of Tetrahydrofolic Acid, Dihydrofolate Reductase, and Homocysteine Pubmed:27064332 |
Cross-sectional Association Between Vitamin B12 Status and Probable Postpartum Depression in Indian Women | |
Int J Biol Macromol | Enhanced cellular uptake, transport and oral bioavailability of optimized folic acid-loaded chitosan nanoparticles Pubmed:35292282 |
Catalog No. | Related products for research use of Pan-species (General) Organism species | Applications (RESEARCH USE ONLY!) |
CPG411Ge11 | BSA Conjugated Tetrahydrofolic Acid (THFA) | Immunogen; SDS-PAGE; WB. |
CPG411Ge21 | OVA Conjugated Tetrahydrofolic Acid (THFA) | Immunogen; SDS-PAGE; WB. |
PAG411Ge01 | Polyclonal Antibody to Tetrahydrofolic Acid (THFA) | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
CEG411Ge | ELISA Kit for Tetrahydrofolic Acid (THFA) | Enzyme-linked immunosorbent assay for Antigen Detection. |
MEG411Ge | Mini Samples ELISA Kit for Tetrahydrofolic Acid (THFA) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMG411Ge | Multiplex Assay Kit for Tetrahydrofolic Acid (THFA) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
KSG411Ge11 | ELISA Kit DIY Materials for Tetrahydrofolic Acid (THFA) | Main materials for "Do It (ELISA Kit) Yourself". |