≡

Multiplex Assay Kit for Homovanillic Acid (HVA) ,etc. by FLIA (Flow Luminescence Immunoassay)

Homovanilic Acid; HOC6H3(OCH3)CH2COOH; 3-Methoxy-4-hydroxyphenyl Acetic Acid; 4-Hydroxy-3-Methoxy-Benzeneacetic Acid; 4-Hydroxy-3-Methoxyphenylacetic Acid

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Homovanillic Acid (HVA) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Homovanillic Acid (HVA) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of Homovanillic Acid (HVA) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Homovanillic Acid (HVA) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 90-97 94
EDTA plasma(n=5) 86-95 90
heparin plasma(n=5) 83-99 93
sodium citrate plasma(n=5) 90-104 94

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Homovanillic Acid (HVA) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Homovanillic Acid (HVA) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Homovanillic Acid (HVA) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 94-105% 79-95% 95-103% 86-98%
EDTA plasma(n=5) 87-101% 81-94% 84-98% 93-105%
heparin plasma(n=5) 95-102% 80-94% 93-104% 80-88%
sodium citrate plasma(n=5) 96-104% 84-105% 93-101% 98-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:HVA) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
    add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Psychopharmacology Ningdong granule: a complementary and alternative therapy in the treatment of attention deficit/hyperactivity disorder PubMed: 21416235
Neuropsychiatr Dis Treat Catha edulis chewing effects on treatment of paranoid schizophrenic patients PubMed: 25926735
Evidence-Based Complementary and Alternative Medicine Ningdong Granule Upregulates the Striatal DA Transporter and Attenuates Stereotyped Behavior of Tourette Syndrome in Rats Pubmed: 33005197
Catalog No. Related products for research use of Pan-species (General) Organism species Applications (RESEARCH USE ONLY!)
CPD717Ge11 BSA Conjugated Homovanillic Acid (HVA) Immunogen; SDS-PAGE; WB.
CPD717Ge21 OVA Conjugated Homovanillic Acid (HVA) Immunogen; SDS-PAGE; WB.
PAD717Ge01 Polyclonal Antibody to Homovanillic Acid (HVA) WB; IHC; ICC; IP.
CED717Ge ELISA Kit for Homovanillic Acid (HVA) Enzyme-linked immunosorbent assay for Antigen Detection.
LMD717Ge Multiplex Assay Kit for Homovanillic Acid (HVA) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSD717Ge11 ELISA Kit DIY Materials for Homovanillic Acid (HVA) Main materials for "Do It (ELISA Kit) Yourself".