Packages (Simulation)
Reagent Preparation
Image (I)
-
Results demonstration
Image (II)
-
Typical Standard Curve
Quality Guarantee
Instruction Manual
Certificate
Multiplex Assay Kit for Synaptosomal Associated Protein 25kDa (SNAP25) ,etc. by FLIA (Flow Luminescence Immunoassay)
RIC4; SEC9; SNAP; SUP; Super protein; Resistance To Inhibitors Of Cholinesterase 4 Homolog
(Note: Up to 8-plex in one testing reaction)
- Product No.LMC955Mi
- Organism SpeciesHomo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Oryctolagus cuniculus (Rabbit), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Equus caballus; Equine (Horse), Chicken (Gallus) Same name, Different species.
- Sample TypeTissue homogenates, cell lysates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3.5h
- Detection Range0.01-10ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.003 ng/mL.
- DownloadInstruction Manual
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
- FOB
US$ 437
US$ 454
US$ 479
US$ 512
US$ 546
US$ 596
US$ 672
US$ 840
Add to Price Calculator
Result
For more details, please contact local distributors!
Specificity
This assay has high sensitivity for detection of Synaptosomal Associated Protein 25kDa (SNAP25) ,etc. by FLIA (Flow Luminescence Immunoassay).
100% cross-reactivity of Synaptosomal Associated Protein 25kDa (SNAP25) ,etc. by FLIA (Flow Luminescence Immunoassay) was observed among Human, Mouse, Rat, Rabbit, Porcine, Bovine, Equine, Gallus.
Recovery
Matrices listed below were spiked with certain level of recombinant Synaptosomal Associated Protein 25kDa (SNAP25) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Synaptosomal Associated Protein 25kDa (SNAP25) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 92-101 | 97 |
| EDTA plasma(n=5) | 86-95 | 91 |
| heparin plasma(n=5) | 90-104 | 97 |
| sodium citrate plasma(n=5) | 87-101 | 93 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Synaptosomal Associated Protein 25kDa (SNAP25) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Synaptosomal Associated Protein 25kDa (SNAP25) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Synaptosomal Associated Protein 25kDa (SNAP25) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 84-101% | 95-104% | 89-101% | 84-92% |
| EDTA plasma(n=5) | 90-99% | 97-104% | 93-101% | 99-105% |
| heparin plasma(n=5) | 87-94% | 78-103% | 98-105% | 80-97% |
| sodium citrate plasma(n=5) | 86-97% | 89-104% | 84-94% | 88-103% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:SNAP25) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
GIVEAWAYS
INCREMENT SERVICES
| Magazine | Citations |
| Journal of Alzheimers Disease | Stabilized Low-n Amyloid-β Oligomers Induce Robust Novel Object Recognition Deficits Associated with Inflammatory, Synaptic, and GABAergic Dysfunction in the … Pubmed:29439327 |
| Scientific Reports | A Rationally Designed Humanized Antibody Selective for Amyloid Beta Oligomers in Alzheimer's Disease Pubmed: 31285517 |
| Catalog No. | Related products for research use of Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Oryctolagus cuniculus (Rabbit), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Equus caballus; Equine (Horse), Chicken (Gallus) Organism species | Applications (RESEARCH USE ONLY!) |
| RPC955Mi01 | Recombinant Synaptosomal Associated Protein 25kDa (SNAP25) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC955Mi01 | Polyclonal Antibody to Synaptosomal Associated Protein 25kDa (SNAP25) | WB; IHC; ICC; IP. |
| MAC955Mi21 | Monoclonal Antibody to Synaptosomal Associated Protein 25kDa (SNAP25) | WB; IHC; ICC; IP. |
| SEC955Mi | ELISA Kit for Synaptosomal Associated Protein 25kDa (SNAP25) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC955Mi | Multiplex Assay Kit for Synaptosomal Associated Protein 25kDa (SNAP25) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |