Multiplex Assay Kit for Apolipoprotein M (APOM) ,etc. by FLIA (Flow Luminescence Immunoassay)

Apo-M; G3a; NG20; Protein G3a

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Apolipoprotein M (APOM) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Apolipoprotein M (APOM) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Apolipoprotein M (APOM) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Apolipoprotein M (APOM) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 96-104 101
EDTA plasma(n=5) 91-98 94
heparin plasma(n=5) 78-92 88

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apolipoprotein M (APOM) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apolipoprotein M (APOM) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Apolipoprotein M (APOM) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-95% 90-104% 78-93% 78-93%
EDTA plasma(n=5) 88-97% 89-96% 80-91% 78-96%
heparin plasma(n=5) 95-105% 91-101% 78-91% 79-94%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:APOM) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
Joint Bone Spine Apolipoprotein m (APOM) levels and APOM rs805297 G/T polymorphism are associated with increased risk ofrheumatoid arthritis Pubmed: 23660425
Apoptosis The miR-573/apoM/Bcl2A1-dependent signal transduction pathway is essential for hepatocyte apoptosis and hepatocarcinogenesis PubMed: 26201458
Lipids in Health and Disease Effects of hyperlipidaemia on plasma apolipoprotein M levels in patients with type 2 diabetes mellitus: an independent case–control study pubmed:27633510
Lipids in Health and Disease A single-nucleotide polymorphism C-724 /del in the proter region of the apolipoprotein M gene is associated with type 2 diabetes mellitus pubmed:27576735
clinica chimica acta ApoM/HDL-C and apoM/apoA-I ratios are indicators of diabetic nephropathy in healthy controls and type 2 diabetes mellitus. pubmed:28073663
International Journal of Clinical and Experimental Pathology Level of serum apolipoprotein m and its diagnostic significance in inflammatory bowel disease files:ijcep0033416.pdf
Scandinavian Journal of Medicine & Science in Sports Endurance training selectively increases HDL-bound sphingosine-1-phosphate in the plasma pubmed:28493600
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY HDL-S1P Protects Endothelial Function and Reduces Lung Injury During Sepsis in vivo and in vitro Pubmed: 32750426
Biology-Basel Biomechanical Properties of Blood Plasma Extracellular Vesicles Revealed by Atomic Force Microscopy 33374530
researchsquare Apolipoprotein M in High Density Lipoprotein Protects Against Astrocyte Apoptosis Induced By Ischemic Insult Via Sphingosine 1-Phosphate Signaling
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPC299Mu01 Recombinant Apolipoprotein M (APOM) Positive Control; Immunogen; SDS-PAGE; WB.
APC299Mu01 Active Apolipoprotein M (APOM) Cell culture; Activity Assays.
PAC299Mu01 Polyclonal Antibody to Apolipoprotein M (APOM) WB; IHC; ICC; IP.
LAC299Mu71 Biotin-Linked Polyclonal Antibody to Apolipoprotein M (APOM) WB; IHC; ICC.
SEC299Mu ELISA Kit for Apolipoprotein M (APOM) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC299Mu Multiplex Assay Kit for Apolipoprotein M (APOM) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.