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Multiplex Assay Kit for 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) ,etc. by FLIA (Flow Luminescence Immunoassay)
11DH; 11-beta-HSD1; HDL; HSD11; HSD11B; HSD11L; SDR26C1; Corticosteroid 11-beta-dehydrogenase isozyme 1; Short Chain Dehydrogenase/Reductase Family 26C,Member 1
(Note: Up to 8-plex in one testing reaction)
- Product No.LMC268Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeTissue homogenates, cell lysates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3.5h
- Detection Range0.1-100ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.033 ng/mL.
- DownloadInstruction Manual
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
- FOB
US$ 437
US$ 454
US$ 479
US$ 512
US$ 546
US$ 596
US$ 672
Result
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Specificity
This assay has high sensitivity and excellent specificity for detection of 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 78-99 | 94 |
EDTA plasma(n=5) | 80-103 | 95 |
heparin plasma(n=5) | 98-105 | 101 |
sodium citrate plasma(n=5) | 98-105 | 102 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 96-103% | 94-101% | 79-97% | 81-101% |
EDTA plasma(n=5) | 85-103% | 83-91% | 80-97% | 92-105% |
heparin plasma(n=5) | 94-101% | 92-101% | 88-103% | 86-93% |
sodium citrate plasma(n=5) | 78-93% | 96-105% | 98-105% | 94-101% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
96-well plate | 1 | Plate sealer for 96 wells | 4 |
Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
Pre-Mixed Magnetic beads (22#:HSD11b1) | 1 | Analysis buffer | 1×20mL |
Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
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Magazine | Citations |
Journal of Endocrinology | Uninephrectomy reduces 11β-hydroxysteroid dehydrogenase type 1 and type 2 concomitantly with an increase in blood pressure in rats PubMed: 22739210 |
Evid Based Complement Alternat Med | Anti-Inflammatory Property of PubMed: 26300946 |
Molecules | Piper sarmentosum Effects on 11β-Hydroxysteroid Dehydrogenase Type 1 Enzyme in Serumand Bone in Rat Model of Glucocorticoid-Induced Osteoporosis. pubmed:27854305 |
Bioorganic Chemistry | A novel derivatives of thiazol-4 (5H)-one and their activity in the inhibition of 11β-hydroxysteroid dehydrogenase type 1 Pubmed:29738970 |
Malaysian Journal of Medical Sciences | An Evidence-Based Review: The Effects of Malaysian Traditional Herbs on Osteoporotic Rat Models |
2.583 | Application of ELISA Technique and Human Microsomes in the Search for 11β-Hydroxysteroid Dehydrogenase Inhibitors Doi: BioMed Research International |
Chemistry & Biodiversity | Synthesis of the N‐methyl derivatives of 2‐aminothiazol‐4 (5H)‐one and their interactions with 11βHSD1‐molecular modeling and in vitro studies Pubmed: 31012543 |
Oxidative Medicine and Cellular Longevity | Hepatoprotective Effects of Morinda citrifolia Leaf Extract on Ovariectomized Rats Fed with Thermoxidized Palm Oil Diet: Evidence at Histological and Ultrastructural … Pubmed: 31827717 |
Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
RPC268Hu01 | Recombinant 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAC268Hu01 | Polyclonal Antibody to 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) | WB; IHC; ICC; IP. |
MAC268Hu22 | Monoclonal Antibody to 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) | WB; IHC; ICC; IP. |
SEC268Hu | ELISA Kit for 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMC268Hu | Multiplex Assay Kit for 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |