≡

Multiplex Assay Kit for Ki-67 Protein (Ki-67) ,etc. by FLIA (Flow Luminescence Immunoassay)

MKI67; KIA; Ki67P; Antigen Identified By Monoclonal Antibody Ki-67

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Ki-67 Protein (Ki-67) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Ki-67 Protein (Ki-67) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Ki-67 Protein (Ki-67) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Ki-67 Protein (Ki-67) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 78-101 97
EDTA plasma(n=5) 86-93 89
heparin plasma(n=5) 94-101 98
sodium citrate plasma(n=5) 94-102 98

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ki-67 Protein (Ki-67) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ki-67 Protein (Ki-67) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ki-67 Protein (Ki-67) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 94-101% 98-105% 98-105% 79-90%
EDTA plasma(n=5) 82-94% 80-99% 80-96% 78-101%
heparin plasma(n=5) 82-89% 88-104% 94-101% 92-105%
sodium citrate plasma(n=5) 96-103% 84-91% 78-97% 96-104%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:Ki-67) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
BMC Research Notes Expressions and clinical significances of c-MET, p-MET and E2f-1 in human gastric carcinoma Pubmed: 24393368
BMC Research Notes Expressions and clinical significances of c-MET, p-MET and E2f-2 in human gastric carcinoma Pubmed:24393368
Chemistry & Biodiversity Chemical composition of aqueous‐ethanol extract of leaves and its effect on representation of caspase 8, caspase 3 and the proliferation marker Ki67 in intrinsic … Pubmed:29874411
Predictive value of the proliferation marker Ki-67 in patients with acute leukemia undergoing hematopoietic stem cell transplantation
Carbohydrate Polymers A novel exopolysaccharide from marine bacterium Pantoea sp. YU16-S3 accelerates cutaneous wound healing through Wnt/β-catenin pathway Pubmed: 32299547
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPC047Mu01 Recombinant Ki-67 Protein (Ki-67) Positive Control; Immunogen; SDS-PAGE; WB.
RPC047Mu02 Recombinant Ki-67 Protein (Ki-67) Positive Control; Immunogen; SDS-PAGE; WB.
PAC047Mu02 Polyclonal Antibody to Ki-67 Protein (Ki-67) WB; IHC; ICC; IP.
PAC047Mu01 Polyclonal Antibody to Ki-67 Protein (Ki-67) WB; IHC; ICC; IP.
SEC047Mu ELISA Kit for Ki-67 Protein (Ki-67) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC047Mu Multiplex Assay Kit for Ki-67 Protein (Ki-67) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSC047Mu01 ELISA Kit DIY Materials for Ki-67 Protein (Ki-67) Main materials for "Do It (ELISA Kit) Yourself".