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![Multiplex Assay Kit for Hemojuvelin (HJV) ,etc. by FLIA (Flow Luminescence Immunoassay)](/upload/static/product/LM.jpg)
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Multiplex Assay Kit for Hemojuvelin (HJV) ,etc. by FLIA (Flow Luminescence Immunoassay)
HFE2; HFE2A; HJV; JH; RGMC; hemochromatosis type 2(Juvenile); Haemojuvelin; RGM domain family member C
(Note: Up to 8-plex in one testing reaction)
- Product No.LMB995Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length1.5h
- Detection Range0.98-1000ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.327 ng/mL.
- DownloadInstruction Manual
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
- FOB
US$ 424
US$ 441
US$ 465
US$ 498
US$ 530
US$ 579
US$ 653
Result
For more details, please contact local distributors! US$ 816 Add to Price Calculator
Specificity
This assay has high sensitivity and excellent specificity for detection of Hemojuvelin (HJV) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Hemojuvelin (HJV) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Hemojuvelin (HJV) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Hemojuvelin (HJV) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 88-98 | 94 |
EDTA plasma(n=5) | 80-97 | 87 |
heparin plasma(n=5) | 95-103 | 98 |
sodium citrate plasma(n=5) | 86-101 | 93 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hemojuvelin (HJV) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hemojuvelin (HJV) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Hemojuvelin (HJV) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 96-105% | 79-99% | 78-104% | 81-98% |
EDTA plasma(n=5) | 90-98% | 93-102% | 84-96% | 87-102% |
heparin plasma(n=5) | 86-95% | 89-99% | 86-102% | 90-101% |
sodium citrate plasma(n=5) | 98-105% | 98-105% | 90-101% | 89-101% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
96-well plate | 1 | Plate sealer for 96 wells | 4 |
Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
Pre-Mixed Magnetic beads (22#:HJV) | 1 | Analysis buffer | 1×20mL |
Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
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Magazine | Citations |
PLoS One. | Effect of Iron Overload and Iron Deficiency on Liver Hemojuvelin Protein PubMed: PMC3356351 |
Journal of | Hepcidin Levels Are Increased in Patients with Acute Ischemic Stroke: Preliminary Report PubMed: 25881778 |
European Journal of Haematology | Soluble hemojuvelin in transfused and untransfused thalassaemic subjects doi:10.1111 |
American Journal of Nephrology | The secret war against counterfeit science pubmed:28492262 |
Critical Care | Urinary biomarkers predict advanced acute kidney injury after cardiovascular surgery Pubmed:29699579 |
Scientific Reports | Hemojuvelin Predicts Acute Kidney Injury and Poor Outcomes Following Cardiac Surgery Pubmed:29386545 |
Journal of Cerebral Blood Flow and Metabolism | The functional role of hemojuvelin in acute ischemic stroke Pubmed: 31307288 |
Oxidative damage and iron dyshomeostasis in thalassemic patients | |
J Trace Elem Med Biol | Caffeine and alcohol-friends or foes of human iron stores? Pubmed:35063815 |
Arch Pathol Lab Med | Urinary Biomarkers Can Predict Weaning From Acute Dialysis Therapy in Critically Ill Patients Pubmed:35311928 |
Arch Pathol Lab Med | The Relationships among Plasma Fetuin-B, Thyroid Autoimmunity, and Fertilization Rate In Vitro Fertilization and Embryo Transfer Pubmed:35340679 |
Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
RPB995Hu03 | Recombinant Hemojuvelin (HJV) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPB995Hu02 | Recombinant Hemojuvelin (HJV) | Positive Control; Immunogen; SDS-PAGE; WB. |
EPB995Hu61 | Eukaryotic Hemojuvelin (HJV) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPB995Hu01 | Recombinant Hemojuvelin (HJV) | Positive Control; Immunogen; SDS-PAGE; WB. |
APB995Hu61 | Active Hemojuvelin (HJV) | Cell culture; Activity Assays. |
RPB995Hu04 | Recombinant Hemojuvelin (HJV) | Positive Control; Immunogen; SDS-PAGE; WB. |
CPB995Hu22 | OVA Conjugated Hemojuvelin (HJV) | Immunogen; SDS-PAGE; WB. |
CPB995Hu21 | OVA Conjugated Hemojuvelin (HJV) | Immunogen; SDS-PAGE; WB. |
PAB995Hu04 | Polyclonal Antibody to Hemojuvelin (HJV) | WB; IHC |
PAB995Hu06 | Polyclonal Antibody to Hemojuvelin (HJV) | WB; IHC; ICC; IP. |
PAB995Hu01 | Polyclonal Antibody to Hemojuvelin (HJV) | WB |
PAB995Hu03 | Polyclonal Antibody to Hemojuvelin (HJV) | WB; IHC |
PAB995Hu02 | Polyclonal Antibody to Hemojuvelin (HJV) | WB; IHC; ICC; IP. |
PAB995Hu08 | Polyclonal Antibody to Hemojuvelin (HJV) | WB; IHC; ICC; IP. |
LAB995Hu81 | FITC-Linked Polyclonal Antibody to Hemojuvelin (HJV) | WB; IHC; ICC; IF. |
LAB995Hu71 | Biotin-Linked Polyclonal Antibody to Hemojuvelin (HJV) | WB; IHC; ICC. |
MAB995Hu28 | Monoclonal Antibody to Hemojuvelin (HJV) | WB; IHC; ICC; IP. |
MAB995Hu24 | Monoclonal Antibody to Hemojuvelin (HJV) | WB |
MAB995Hu27 | Monoclonal Antibody to Hemojuvelin (HJV) | WB; IHC; ICC; IP. |
MAB995Hu29 | Monoclonal Antibody to Hemojuvelin (HJV) | WB; IHC; ICC; IP. |
MAB995Hu26 | Monoclonal Antibody to Hemojuvelin (HJV) | WB; IHC; ICC; IP. |
MAB995Hu22 | Monoclonal Antibody to Hemojuvelin (HJV) | WB |
MAB995Hu23 | Monoclonal Antibody to Hemojuvelin (HJV) | WB |
MAB995Hu25 | Monoclonal Antibody to Hemojuvelin (HJV) | WB; IHC; ICC; IP. |
MAB995Hu21 | Monoclonal Antibody to Hemojuvelin (HJV) | WB; IHC; ICC; IP. |
LAB995Hu74 | Biotin-Linked Monoclonal Antibody to Hemojuvelin (HJV) | WB; IHC; ICC. |
LAB995Hu48 | PE-Linked Monoclonal Antibody to Hemojuvelin (HJV) | WB; IHC; ICC; IF. |
LAB995Hu76 | Biotin-Linked Monoclonal Antibody to Hemojuvelin (HJV) | WB; IHC; ICC. |
SEB995Hu | ELISA Kit for Hemojuvelin (HJV) | Enzyme-linked immunosorbent assay for Antigen Detection. |
CEB995Hu | ELISA Kit for Hemojuvelin (HJV) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB995Hu | Multiplex Assay Kit for Hemojuvelin (HJV) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
PSB995Hu01 | Antibody Pair for Hemojuvelin (HJV) | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
KSB995Hu01 | ELISA Kit DIY Materials for Hemojuvelin (HJV) | Main materials for "Do It (ELISA Kit) Yourself". |