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Multiplex Assay Kit for Fetuin B (FETUB) ,etc. by FLIA (Flow Luminescence Immunoassay)

FETU-B; 16G2; Gugu; IRL685; Fetuin-like protein IRL685

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Fetuin B (FETUB) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Fetuin B (FETUB) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Fetuin B (FETUB) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Fetuin B (FETUB) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 83-97 87
EDTA plasma(n=5) 80-90 86
heparin plasma(n=5) 89-103 99

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fetuin B (FETUB) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fetuin B (FETUB) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fetuin B (FETUB) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-95% 86-96% 81-104% 93-101%
EDTA plasma(n=5) 86-101% 98-105% 79-102% 98-105%
heparin plasma(n=5) 82-102% 95-103% 81-88% 86-95%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:FETUB) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
Endocrine Serum fetuin B level increased in subjects of nonalcoholic fatty liver disease: a case-control study pubmed:27628583
Endocrine Increased serum levels of fetuin B in patients with coronary artery disease pubmed:28822077
DIABETES & METABOLISM Plasma fetuin-B concentrations are associated with insulin resistance and first-phase glucose-stimulated insulin secretion in individuals with different degrees of … Pubmed:29514765
Clinical & Investigative Medicine Serum level of fetuin B is associated with osteoporosis: a 4-year prospective study in China Pubmed:29603688
American Journal of Translational Research Fetuin B aggravates liver X receptor-mediated hepatic steatosis through AMPK in HepG2 cells and mice Pubmed: 30972177
Endocrine Connections Increased serum fetuin-B concentration is associated with HOMA-β and indices of liver steatosis in women with polycystic ovary syndrome: a pilot study Pubmed: 31307012
J Transl Med Multi-omic signatures of atherogenic dyslipidaemia: pre-clinical target identification and validation in humans 33407555
J Nephrol Citrate high volume on-line hemodiafiltration modulates serum Interleukin-6 and Klotho levels: the multicenter randomized controlled study ¡°Hephaestus¡± 33559851
Nutrients High Fat, High Sugar Diet and DJOS Bariatric Surgery Influence Plasma Levels of Fetuin-B, Growth Differentiation Factor-15, and Pentraxin 3 in Diet-Induced Obese … 34684637
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB860Hu01 Recombinant Fetuin B (FETUB) Positive Control; Immunogen; SDS-PAGE; WB.
PAB860Hu01 Polyclonal Antibody to Fetuin B (FETUB) WB; IHC; ICC; IP.
LAB860Hu81 FITC-Linked Polyclonal Antibody to Fetuin B (FETUB) WB; IHC; ICC; IF.
LAB860Hu71 Biotin-Linked Polyclonal Antibody to Fetuin B (FETUB) WB; IHC; ICC.
MAB860Hu22 Monoclonal Antibody to Fetuin B (FETUB) WB; IHC; ICC; IP.
SEB860Hu ELISA Kit for Fetuin B (FETUB) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB860Hu Multiplex Assay Kit for Fetuin B (FETUB) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.