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Multiplex Assay Kit for Lipocalin 1 (LCN1) ,etc. by FLIA (Flow Luminescence Immunoassay)

TP; PMFA; VEGP; Tlc; Tear Prealbumin; Tear lipocalin; Protein Migrating Faster Than Albumin; Von Ebner Gland Protein

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Lipocalin 1 (LCN1) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Lipocalin 1 (LCN1) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Lipocalin 1 (LCN1) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Lipocalin 1 (LCN1) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 81-101 92
EDTA plasma(n=5) 98-105 102
heparin plasma(n=5) 98-105 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lipocalin 1 (LCN1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lipocalin 1 (LCN1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Lipocalin 1 (LCN1) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 94-101% 80-92% 81-94% 94-101%
EDTA plasma(n=5) 98-105% 90-97% 78-99% 99-105%
heparin plasma(n=5) 98-105% 80-90% 98-105% 95-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:LCN1) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
Fertility and Sterility Lipocalin-1: a potential marker for noninvasive aneuploidy screening ScienceDirect: S0015028211001750
PROTEOMICS-Clinical Applications Proteomic analysis of the aqueous humor in patients with wet age-related macular degeneration Pubmed: 23418058
International Journal of Sport Studies Lipocalin-2: Response To Resistance Training in Obese Young Men Ijssjournal: Source
Int J Chron Obstruct Pulmon Dis. Increased serum levels of lipocalin-1 and-2 in patients with stable chronic obstructive pulmonary disease Pubmed:Pmc4043430
Scientific Reports The basal free fatty acid concentration in human saliva is related to salivary lipolytic activity pubmed:28729703
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPB706Ra01 Recombinant Lipocalin 1 (LCN1) Positive Control; Immunogen; SDS-PAGE; WB.
PAB706Ra01 Polyclonal Antibody to Lipocalin 1 (LCN1) WB; IHC; ICC; IP.
SEB706Ra ELISA Kit for Lipocalin 1 (LCN1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB706Ra Multiplex Assay Kit for Lipocalin 1 (LCN1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB706Ra01 ELISA Kit DIY Materials for Lipocalin 1 (LCN1) Main materials for "Do It (ELISA Kit) Yourself".