Multiplex Assay Kit for Prominin 1 (PROM1) ,etc. by FLIA (Flow Luminescence Immunoassay)

CD133; PROML1; Macular Dystrophy,Retinal 2; Stargardt Disease 4(Autosomal Dominant); Prominin-like protein 1; Antigen AC133

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Prominin 1 (PROM1) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Prominin 1 (PROM1) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Prominin 1 (PROM1) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Prominin 1 (PROM1) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 85-97 91
EDTA plasma(n=5) 85-103 88
heparin plasma(n=5) 98-105 102

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prominin 1 (PROM1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prominin 1 (PROM1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Prominin 1 (PROM1) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-92% 95-104% 98-105% 90-98%
EDTA plasma(n=5) 87-103% 78-90% 78-91% 91-98%
heparin plasma(n=5) 88-97% 79-98% 91-98% 97-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:PROM1) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
J Thorac Cardiovasc Surg Clinical and biological significance of stem-like CD133+ CXCR4+ cells in esophageal squamous cell carcinoma PubMed: 26092504
Journal of Histochemistry & Cytochemistry Hyperglycemia Alters the Protein Levels of Prominin-1 and VEGFA in the Retina of Albino Rats pubmed:29076766
Analyst Matter, energy and information network of graphene-peptide-based fluorescent sensing system for molecular logic computing, detection and imaging of cancer stem?… Pubmed: 30785136
Advanced Healthcare Materials Impact of Hydrogel Elasticity and Adherence on Osteosarcoma Cells and Osteoblasts Pubmed: 30838809
JOURNAL OF CANCER Prognostic value of stem cell markers in esophageal and esophagogastric junction cancer: a meta-analysis Pubmed: 32368307
Expression of CD44 and CD133 markers in regional metastases from breast cancer.
Materials Antibody CD133 Biofunctionalization of Ammonium Acryloyldimethyltaurate and Vinylpyrrolidone Co-Polymer-Based Coating of the Vascular Implants 33321837
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB516Hu01 Recombinant Prominin 1 (PROM1) Positive Control; Immunogen; SDS-PAGE; WB.
PAB516Hu01 Polyclonal Antibody to Prominin 1 (PROM1) WB; IHC; ICC; IP.
LAB516Hu71 Biotin-Linked Polyclonal Antibody to Prominin 1 (PROM1) WB; IHC; ICC.
LAB516Hu81 FITC-Linked Polyclonal Antibody to Prominin 1 (PROM1) WB; IHC; ICC; IF.
FAB516Hu51 APC-Linked Anti-Prominin 1 (PROM1) Polyclonal Antibody FCM
MAB516Hu22 Monoclonal Antibody to Prominin 1 (PROM1) WB; IHC; ICC; IP.
FAB516Hu02 Anti-Prominin 1 (PROM1) Monoclonal Antibody FCM
LAB516Hu82 FITC-Linked Monoclonal Antibody to Prominin 1 (PROM1) WB; IHC; ICC; IF.
SEB516Hu ELISA Kit for Prominin 1 (PROM1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB516Hu Multiplex Assay Kit for Prominin 1 (PROM1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.