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Multiplex Assay Kit for Pancreatic Lipase (PL) ,etc. by FLIA (Flow Luminescence Immunoassay)

PNLIP; PTL; Pancreatic Lipase; Pancreatic triacylglycerol lipase

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Pancreatic Lipase (PL) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Pancreatic Lipase (PL) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Pancreatic Lipase (PL) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Pancreatic Lipase (PL) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 89-98 93
EDTA plasma(n=5) 78-101 83
heparin plasma(n=5) 96-105 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pancreatic Lipase (PL) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pancreatic Lipase (PL) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pancreatic Lipase (PL) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-103% 82-96% 98-105% 89-102%
EDTA plasma(n=5) 95-103% 99-105% 87-95% 91-104%
heparin plasma(n=5) 90-97% 96-105% 84-94% 95-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:PL) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
Toxicol Pathol.? Mechanistic investigations of test?article-induced?pancreatic toxicity at the endocrine-exocrine interface in the rat. Pubmed:24226507
The Korean Journal of Internal Medicine Clinical efficacy of serum lipase subtype analy-sis for the differential diagnosis of pancreatic and non-pancreatic lipase elevation Pubmed:27243230
Clinical Nutrition ESPEN Abdominal fat distribution modulates the metabolic effects of exogenous ketones in individuals with new-onset prediabetes after acute pancreatitis: Results from a randomized placebo-controlled trial 34024503
Obes Facts Associations between Intra-Pancreatic Fat Deposition, Pancreas Size, and Pancreatic Enzymes in Health and after an Attack of Acute Pancreatitis 34753126
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB453Hu01 Recombinant Pancreatic Lipase (PL) Positive Control; Immunogen; SDS-PAGE; WB.
PAB453Hu01 Polyclonal Antibody to Pancreatic Lipase (PL) WB; IHC; ICC; IP.
MAB453Hu21 Monoclonal Antibody to Pancreatic Lipase (PL) WB; IHC; ICC; IP.
SEB453Hu ELISA Kit for Pancreatic Lipase (PL) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB453Hu Multiplex Assay Kit for Pancreatic Lipase (PL) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.