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Multiplex Assay Kit for Pleiotrophin (PTN) ,etc. by FLIA (Flow Luminescence Immunoassay)

HARP; HBGF8; HBNF; NEGF1; Heparin Binding Growth Factor 8; Neurite Growth-Promoting Factor 1; Heparin Affin Regulatory Peptide; Heparin Binding Growth Associated Molecule

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Pleiotrophin (PTN) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Pleiotrophin (PTN) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Pleiotrophin (PTN) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Pleiotrophin (PTN) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 95-103 101
EDTA plasma(n=5) 89-96 93
heparin plasma(n=5) 93-102 97
sodium citrate plasma(n=5) 85-103 92

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pleiotrophin (PTN) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pleiotrophin (PTN) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pleiotrophin (PTN) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 82-103% 86-101% 78-94% 89-104%
EDTA plasma(n=5) 78-104% 88-103% 88-105% 93-105%
heparin plasma(n=5) 78-95% 81-97% 95-103% 90-97%
sodium citrate plasma(n=5) 91-98% 78-98% 80-103% 97-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:PTN) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
Oncogene Suppression of lung adenocarcinoma through menin and polycomb gene-mediated repression of growth factor pleiotrophinMenin suppresses lung cancer through PTN Nature: 2009273
PLOS ONE The Effects of Pleiotrophin in Proliferative Diabetic Retinopathy Pubmed:25617851
Infect Immun.  Depletion of Phagocytic Cells during Nonlethal Plasmodium yoelii Infection Causes Severe Malaria Characterized by Acute Renal Failure in Mice. Pubmed:26755155
In Vivo Evaluation of Serum Biomarkers (FGF-2, HGF, MIF and PTN) in Patients With Testicular Germ Cell Cancer Pubmed: 31662522
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPB309Ra01 Recombinant Pleiotrophin (PTN) Positive Control; Immunogen; SDS-PAGE; WB.
PAB309Ra01 Polyclonal Antibody to Pleiotrophin (PTN) WB; IHC; ICC; IP.
MAB309Ra21 Monoclonal Antibody to Pleiotrophin (PTN) WB; IHC; ICC; IP.
SEB309Ra ELISA Kit for Pleiotrophin (PTN) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB309Ra CLIA Kit for Pleiotrophin (PTN) Chemiluminescent immunoassay for Antigen Detection.
LMB309Ra Multiplex Assay Kit for Pleiotrophin (PTN) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.