Multiplex Assay Kit for Transferrin Receptor (TFR) ,etc. by FLIA (Flow Luminescence Immunoassay)

CD71; TFR1; P90; TFRC; T9; Trfr; sTfR; Transferrin receptor protein 1, serum form

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Transferrin Receptor (TFR) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Transferrin Receptor (TFR) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Transferrin Receptor (TFR) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Transferrin Receptor (TFR) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 79-104 86
EDTA plasma(n=5) 89-101 96
heparin plasma(n=5) 89-96 92

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transferrin Receptor (TFR) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transferrin Receptor (TFR) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Transferrin Receptor (TFR) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 95-105% 89-103% 84-103% 79-103%
EDTA plasma(n=5) 98-105% 92-101% 99-105% 92-103%
heparin plasma(n=5) 80-103% 96-103% 84-102% 94-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:TFR) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
British Journal of Nutrition Survey of anaemia and Helicobacter pylori infection in adolescent girls in Suihua, China and enhancement of iron intervention effects by H. pylori eradication PubMed: 22004585
Food Funct The effects of polysaccharides from the root of Angelica sinensis on tumor growth and iron metabolism in H22-bearing mice Pubmed:26757699
Physiological research The Labile Iron Pool in Monocytes Reflects the Activity of the Atherosclerotic Process in Men with Chronic Cardiovascular Disease pubmed:27782743
Clinical and Experimental Medicine Increased NGAL level associated with iron store in chronic kidney disease with anemia Pubmed:29909502
J Mol Cell Cardiol Systemic iron deficiency does not affect the cardiac iron content and progression of heart failure 34139233
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APB171Hu62 Active Transferrin Receptor (TFR) Cell culture; Activity Assays.
APB171Hu61 Active Transferrin Receptor (TFR) Cell culture; Activity Assays.
EPB171Hu61 Eukaryotic Transferrin Receptor (TFR) Positive Control; Immunogen; SDS-PAGE; WB.
RPB171Hu01 Recombinant Transferrin Receptor (TFR) Positive Control; Immunogen; SDS-PAGE; WB.
EPB171Hu62 Eukaryotic Transferrin Receptor (TFR) Positive Control; Immunogen; SDS-PAGE; WB.
PAB171Hu01 Polyclonal Antibody to Transferrin Receptor (TFR) WB; IHC
CAB171Hu01 Anti-Transferrin Receptor (TFR) Polyclonal Antibody Loading Control of WB.
LAB171Hu81 FITC-Linked Polyclonal Antibody to Transferrin Receptor (TFR) WB; IHC; ICC; IF.
LAB171Hu71 Biotin-Linked Polyclonal Antibody to Transferrin Receptor (TFR) WB; IHC; ICC.
CAB171Hu24 Anti-Transferrin Receptor (TFR) Monoclonal Antibody Loading Control of WB.
MAB171Hu22 Monoclonal Antibody to Transferrin Receptor (TFR) IHC
MAB171Hu26 Monoclonal Antibody to Transferrin Receptor (TFR) WB
MAB171Hu23 Monoclonal Antibody to Transferrin Receptor (TFR) WB; IHC; ICC; IP.
MAB171Hu24 Monoclonal Antibody to Transferrin Receptor (TFR) WB; IHC; ICC; IP.
MAB171Hu25 Monoclonal Antibody to Transferrin Receptor (TFR) WB
MAB171Hu21 Monoclonal Antibody to Transferrin Receptor (TFR) WB; IHC; ICC; IP.
CAB171Hu22 Anti-Transferrin Receptor (TFR) Monoclonal Antibody Loading Control of WB.
FAB171Hu02 Anti-Transferrin Receptor (TFR) Monoclonal Antibody Flow cytometry.
FAB171Hu86 FITC-Linked Anti-Transferrin Receptor (TFR) Monoclonal Antibody FCM
FAB171Hu42 PE-Linked Anti-Transferrin Receptor (TFR) Monoclonal Antibody FCM
SEB171Hu ELISA Kit for Transferrin Receptor (TFR) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB171Hu Multiplex Assay Kit for Transferrin Receptor (TFR) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.