≡

Multiplex Assay Kit for Galanin (GAL) ,etc. by FLIA (Flow Luminescence Immunoassay)

GALN; GLNN; GMAP; Galanin Prepropeptide; Galanin-Message-Associated Peptide

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Galanin (GAL) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Galanin (GAL) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Galanin (GAL) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Galanin (GAL) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 95-105 98
EDTA plasma(n=5) 96-103 99
heparin plasma(n=5) 90-104 96
sodium citrate plasma(n=5) 84-92 89

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Galanin (GAL) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Galanin (GAL) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Galanin (GAL) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-104% 94-102% 85-93% 88-98%
EDTA plasma(n=5) 94-102% 96-105% 82-102% 80-102%
heparin plasma(n=5) 85-102% 85-101% 79-104% 78-105%
sodium citrate plasma(n=5) 84-93% 78-99% 79-91% 79-93%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:GAL) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
    add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Progress in Neuro-Psychopharmacology and Biological Psychiatry Decreased galanin serum levels are associated with alcohol-craving during withdrawal ScienceDirect: S0278584610005191
Clinical Biochemistry Circulating galanin levels are increased in patients with gestational diabetes mellitus PubMed: 23266294
Peptides Endogenous galanin as a novel biomarker to predict gestational diabetes mellitus. Pubmed: 24503374
Gynecological Endocrinology Circulating galanin and IL-6 concentrations in gestational diabetes mellitus Pubmed:24397394
Eat Behav Association between circulating levels of galanin and pre-pregnancy body mass index in patients with gestational diabetes mellitus PubMed: 26172564
Cell Biochemistry and Biophysics The Effects of Levetiracetam on Cerebrospinal Fluid and Plasma NPY and GAL, and on the Components of Stress Response System, hs-CRP, and S100B Protein in Serum of Patients with Refractory Epilepsy Article: 10.1007
Journal of Addiction & Prevention Low Galanin Serum Levels are Associated with Anxiety in Opioid-Maintained Patients 688687ca8d5a1d03ed060f761328001ca673.pdf
Oncology Letter Colorectal cancer patients exhibit increased levels of galanin in serum and colon tissues pubmed:27899999
Scientific Reports Serum Galanin Levels in Young Healthy Lean and Obese Non-Diabetic Men during an OralGlucose Tolerance Test. pubmed:27550417
Journal of Clinical Investigation  Adrenomedullin and Galanin Responses to Orthostasis in Older Persons pubmed:28796366
Child Psychiatry Hum Dev Serum Galanin in Children with Autism Spectrum Disorder 33534034
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPB084Ra01 Recombinant Galanin (GAL) Positive Control; Immunogen; SDS-PAGE; WB.
PAB084Ra01 Polyclonal Antibody to Galanin (GAL) WB; IHC; ICC; IP.
MAB084Ra21 Monoclonal Antibody to Galanin (GAL) WB; IHC; ICC; IP.
CEB084Ra ELISA Kit for Galanin (GAL) Enzyme-linked immunosorbent assay for Antigen Detection.
CCB084Ra CLIA Kit for Galanin (GAL) Chemiluminescent immunoassay for Antigen Detection.
LMB084Ra Multiplex Assay Kit for Galanin (GAL) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB084Ra11 ELISA Kit DIY Materials for Galanin (GAL) Main materials for "Do It (ELISA Kit) Yourself".