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Multiplex Assay Kit for Peptide YY (PYY) ,etc. by FLIA (Flow Luminescence Immunoassay)
PYY1; Peptide tyrosine tyrosine
(Note: Up to 8-plex in one testing reaction)
- Product No.LMB067Po
- Organism SpeciesSus scrofa; Porcine (Pig) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length1.5h
- Detection Range0.98-1000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.327 pg/mL.
- DownloadInstruction Manual
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
- FOB
US$ 498
US$ 517
US$ 546
US$ 584
US$ 623
US$ 680
US$ 766
Result
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Specificity
This assay has high sensitivity and excellent specificity for detection of Peptide YY (PYY) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Peptide YY (PYY) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Peptide YY (PYY) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Peptide YY (PYY) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 95-102 | 98 |
EDTA plasma(n=5) | 96-105 | 102 |
heparin plasma(n=5) | 80-94 | 84 |
sodium citrate plasma(n=5) | 88-99 | 96 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peptide YY (PYY) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peptide YY (PYY) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Peptide YY (PYY) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 88-104% | 93-101% | 82-98% | 79-93% |
EDTA plasma(n=5) | 88-96% | 85-99% | 86-98% | 95-102% |
heparin plasma(n=5) | 79-93% | 83-102% | 80-101% | 98-105% |
sodium citrate plasma(n=5) | 88-98% | 80-101% | 90-97% | 89-101% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
96-well plate | 1 | Plate sealer for 96 wells | 4 |
Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
Pre-Mixed Magnetic beads (22#:PYY) | 1 | Analysis buffer | 1×20mL |
Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
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Magazine | Citations |
International Journal of Sport Studies | The Effects of Concurrent Resistance and Endurance Exercise on Hunger Feelings and PYY in Obese Men Com:Source |
Annals of Applied Sport Science | Effect of an Acute Incremental Exercise on Plasma Peptide YY, Neuropeptide Y and IGF-1 Concentrations in Young Athletes Aassjournal:Source |
Biomedical Reports | Calprotectin in serum and zonulin in serum and feces are elevated after introduction of a diet with lower carbohydrate content and higher fiber, fat and protein contents. pubmed:28413639 |
Acta Diabetologica | Effect of lifestyle improvement program on the biomarkersof adiposity, inflammation and gut hormones in overweight/obese Asian Indians with prediabetes pubmed:28620678 |
Biomedical Reports | Calprotectin in serum and zonulin in serum and feces are elevated after introduction of a diet with lower carbohydrate content and higher fiber, fat and protein contents 10.3892/br.2017.865 |
Journal of Diabetes & Metabolism | High Fiber Fat and Protein Contents Lead to Increased Satiety Reduced Sweet Cravings and Decreased Gastrointestinal Symptoms Independently of Anthropometric Hormonal and Metabolic Factors 10.4172/2155-6156.1000733 |
Frontiers in Physiology | Ileal Transposition Surgery Decreases Fat Mass and Improves Glucose Metabolism in Diabetic GK Rats: Possible Involvement of FGF21 Pubmed:29593555 |
Food and Nutrition Research | Alignments of endocrine, anthropometric, and metabolic parameters in type 2 diabetes after intervention with an Okinawa-based Nordic diet Pubmed:29599686 |
The Journal of Nutrition | Grape Seed Proanthocyanidin Affects Lipid Metabolism via Changing Gut Microflora and Enhancing Propionate Production in Weaned Pigs Pubmed: 31175811 |
The Journal of Nutritional Biochemistry | Bile acid receptor TGR5 is critically involved in preference for dietary lipids and obesity Pubmed: 31812910 |
Arch Toxicol | Serotonin involvement in okadaic acid-induced diarrhoea in vivo 34148100 |
foods | Appetite and Satiety Effects of the Acute and Regular Consumption of Green Coffee Phenols and Green Coffee Phenol/Oat β-Glucan Nutraceuticals in Subjects with … 34828792 |
Catalog No. | Related products for research use of Sus scrofa; Porcine (Pig) Organism species | Applications (RESEARCH USE ONLY!) |
RPB067Po01 | Recombinant Peptide YY (PYY) | Positive Control; Immunogen; SDS-PAGE; WB. |
CPB067Po21 | OVA Conjugated Peptide YY (PYY) | Immunogen; SDS-PAGE; WB. |
PAB067Po01 | Polyclonal Antibody to Peptide YY (PYY) | WB; IHC; ICC; IP. |
MAB067Po21 | Monoclonal Antibody to Peptide YY (PYY) | WB; IHC; ICC; IP. |
CEB067Po | ELISA Kit for Peptide YY (PYY) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB067Po | Multiplex Assay Kit for Peptide YY (PYY) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |