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Multiplex Assay Kit for Albumin (ALB) ,etc. by FLIA (Flow Luminescence Immunoassay)
Serum albumin
(Note: Up to 8-plex in one testing reaction)
- Product No.LMB028Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length1.5h
- Detection Range19.53-20000ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 6.51 ng/mL.
- DownloadInstruction Manual
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
- FOB
US$ 348
US$ 362
US$ 382
US$ 409
US$ 436
US$ 476
US$ 536
Result
For more details, please contact local distributors! US$ 670 Add to Price Calculator
Specificity
This assay has high sensitivity and excellent specificity for detection of Albumin (ALB) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Albumin (ALB) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Albumin (ALB) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Albumin (ALB) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 88-101 | 95 |
EDTA plasma(n=5) | 88-102 | 97 |
heparin plasma(n=5) | 97-104 | 101 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Albumin (ALB) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Albumin (ALB) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Albumin (ALB) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 91-101% | 79-93% | 86-101% | 83-95% |
EDTA plasma(n=5) | 84-97% | 89-96% | 89-103% | 96-104% |
heparin plasma(n=5) | 84-92% | 79-102% | 85-99% | 85-98% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
96-well plate | 1 | Plate sealer for 96 wells | 4 |
Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
Pre-Mixed Magnetic beads (22#:ALB) | 1 | Analysis buffer | 1×20mL |
Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
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Magazine | Citations |
PLoS One | Simvastatin Reduces Endotoxin-Induced Acute Lung Injury by Decreasing Neutrophil Recruitment and Radical Formation PubMed: 22701728 |
Liver International | Albumin administration protects against bilirubin-induced auditory brainstem dysfunction in Gunn rat pups Onlinelibrary: liv.12219 |
Journal of Infectious Diseases | Adjunctive corticosteroid therapy improves lung immunopathology and survival during severe secondary pneumococcal pneumonia in mice Pubmed: 24273183 |
Toxins (Basel). | Increased Circulating Levels of Vitamin D Binding Protein in MS Patients Pubmed:25590278 |
Mol Cell Biochem | The wnt/β-catenin signaling pathway participates in rhein ameliorating kidney injury in DN mice PubMed: 26346164 |
PLoS One | Collecting Protein Biomarkers in Breath Using Electret Filters: A Preliminary Method on New Technical Model and Human Study Pubmed:26934615 |
Journal of Biomaterials and Tissue Engineering | 3D Printing of Differentiated Bone Marrow Mesenchymal Cells as a New Method for Liver Tissue Engineering art00007 |
Experimental and molecular pathology | Alteration of Connexin43 expression in a rat model of obesity-related glomerulopathy. pubmed:29246788 |
Inflammation | Resolvin D1 Promotes SIRT1 Expression to Counteract the Activation of STAT3 and NF-κB in Mice with Septic-Associated Lung Injury Pubmed:30014231 |
Journal of Developmental Origins of Health and Disease | Maternal low-quality protein diet exerts sex-specific effects on plasma amino acid profile and alters hepatic expression of methyltransferases in adult rat offspring Pubmed:29582727 |
Medicine | Electret filter collects more exhaled albumin than glass condenser: A method comparison based on human study Pubmed:29384875 |
Stem Cell Research & Therapy | Therapeutic efficiency of human amniotic epithelial stem cell-derived functional hepatocyte-like cells in mice with acute hepatic failure Pubmed: 30463600 |
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A | Decellularized Human Liver Scaffold‐Based Three‐dimensional Culture System Facilitate HBV Infection Pubmed: 30963688 |
biomedicine & pharmacotherapy | Treatment with 3, 4-dihydroxyphenylethyl alcohol glycoside ameliorates sepsis-induced ALI in mice by reducing inflammation and regulating M1 polarization Pubmed: 31146107 |
biochemical and biophysical research communications | Anti-proteinuria effect of antibody against ANGPTL3 coil-coiled domain on adriamycin-induced nephropathy in mice Pubmed: 31256934 |
Diabetes, Metabolic Syndrome and Obesity | A small molecule inhibitor MCC950 ameliorates kidney injury in diabetic nephropathy by inhibiting NLRP3 inflammasome activation Pubmed: 31447572 |
Molecular and Cellular Endocrinology | Diiodothyronines regulate metabolic homeostasis in primary human hepatocytes by modulating mTORC1 and mTORC2 activity Pubmed: 31580898 |
BMC Veterinary Research | Preliminary study on the effects of enrofloxacin, flunixin meglumine and pegbovigrastim on Mycoplasma bovis pneumonia Pubmed: 31655595 |
International Immunopharmacology | Ghrelin alleviates traumatic brain injury-induced acute lung injury through pyroptosis/NF-κB pathway Pubmed: 31918060 |
HYPERTENSION RESEARCH | Sodium butyrate ameliorates deoxycorticosterone acetate/salt-induced hypertension and renal damage by inhibiting the MR/SGK1 pathway Pubmed: 32908237 |
Obesity Surgery | Sleeve Gastrectomy Attenuates Diabetic Nephropathy by Upregulating Nephrin Expressions in Diabetic Obese Rats Pubmed: 32399849 |
Protective Role of Training and Selenium Consumption Against Renal Toxicity Induced by Cadmium in Rats | |
Nutrition | Gut Dysbiosis Contribute to High Fructose Induced Salt Sensitive Hypertension In Sprague-Dawley Rats |
sensors and actuators b-chemical | A light-induced self-powered competitive immunosensor for the detection of platelet derived growth factor-BB via an elaborately assembled bioconjugate |
Biochem Biophys Res Commun | cGAS promotes sepsis in radiotherapy of cancer by up-regulating caspase-11 signaling 33721834 |
Journal of Chinese Pharmaceutical Sciences | A simple and sensitive electrochemical impedimetric immunosensor for the detection of apolipoprotein E4 based on CeZnO nanoparticle |
Inflammation | Syringaresinol Resisted Sepsis-Induced Acute Lung Injury by Suppressing Pyroptosis Via the Oestrogen Receptor-β Signalling Pathway 34807349 |
Nat Commun | GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity 34853313 |
ACS Appl Mater Interfaces | Emitter–Quencher Pair of Single Atomic Co Sites and Monolayer Titanium Carbide MXenes for Luminol Chemiluminescent Reactions 34914377 |
Food & Function | Moderation of gut microbiota and bile acid metabolism by chlorogenic acid improves high-fructose-induced salt-sensitive hypertension in mice Pubmed:35708145 |
Authorea | Syringaresinol attenuates kidney injury by inhibition of pyroptosis via activating the Nrf2 antioxidant pathway in diabetic nephropathy |
Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
NPB028Hu01 | Native Albumin (ALB) | Positive Control; Immunogen; SDS-PAGE; WB. |
NPB028Hu02 | Native Albumin (ALB) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPB028Hu01 | Recombinant Albumin (ALB) | Positive Control; Immunogen; SDS-PAGE; WB. |
EPB028Hu51 | Eukaryotic Albumin (ALB) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPB028Hu51 | Recombinant Albumin (ALB) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB028Hu07 | Polyclonal Antibody to Albumin (ALB) | WB; IHC; ICC; IP. |
PAB028Hu05 | Polyclonal Antibody to Albumin (ALB) | WB; IHC; ICC; IP. |
PAB028Hu51 | Polyclonal Antibody to Albumin (ALB) | WB; IHC; ICC; IP. |
PAB028Hu01 | Polyclonal Antibody to Albumin (ALB) | WB; IHC; ICC; IP. |
PAB028Hu02 | Polyclonal Antibody to Albumin (ALB) | WB; IHC; ICC; IP. |
LAB028Hu71 | Biotin-Linked Polyclonal Antibody to Albumin (ALB) | WB; IHC; ICC. |
LAB028Hu81 | FITC-Linked Polyclonal Antibody to Albumin (ALB) | WB; IHC; ICC; IF. |
MAB028Hu23 | Monoclonal Antibody to Albumin (ALB) | WB; IHC; ICC; IP. |
MAB028Hu22 | Monoclonal Antibody to Albumin (ALB) | WB; IHC |
MAB028Hu21 | Monoclonal Antibody to Albumin (ALB) | WB; IHC; ICC; IP. |
HEB028Hu | High Sensitive ELISA Kit for Albumin (ALB) | Enzyme-linked immunosorbent assay for Antigen Detection. |
CEB028Hu | ELISA Kit for Albumin (ALB) | Enzyme-linked immunosorbent assay for Antigen Detection. |
AEB028Hu | ELISA Kit for Anti-Albumin Antibody (Anti-ALB) | Enzyme-linked immunosorbent assay for Antibody Detection. |
ACB028Hu | CLIA Kit for Anti-Albumin Antibody (Anti-ALB) | Chemiluminescent immunoassay for Antibody Detection. |
CCB028Hu | CLIA Kit for Albumin (ALB) | Chemiluminescent immunoassay for Antigen Detection. |
LMB028Hu | Multiplex Assay Kit for Albumin (ALB) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
KSB028Hu11 | ELISA Kit DIY Materials for Albumin (ALB) | Main materials for "Do It (ELISA Kit) Yourself". |