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Multiplex Assay Kit for Noradrenaline (NE) ,etc. by FLIA (Flow Luminescence Immunoassay)
NA; NAd; Norepinephrine; Norepi
(Note: Up to 8-plex in one testing reaction)
- Product No.LMA907Ge
- Organism SpeciesPan-species (General) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length1.5h
- Detection Range4.88-5000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 1.627 pg/mL.
- DownloadInstruction Manual
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
- FOB
US$ 497
US$ 516
US$ 544
US$ 583
US$ 621
US$ 678
US$ 764
Result
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Specificity
This assay has high sensitivity and excellent specificity for detection of Noradrenaline (NE) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Noradrenaline (NE) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of Noradrenaline (NE) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Noradrenaline (NE) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 92-101 | 97 |
EDTA plasma(n=5) | 79-101 | 89 |
heparin plasma(n=5) | 91-98 | 94 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Noradrenaline (NE) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Noradrenaline (NE) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Noradrenaline (NE) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 90-97% | 97-104% | 80-99% | 85-96% |
EDTA plasma(n=5) | 89-101% | 82-101% | 93-101% | 89-96% |
heparin plasma(n=5) | 95-102% | 82-92% | 79-93% | 87-102% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
96-well plate | 1 | Plate sealer for 96 wells | 4 |
Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
Pre-Mixed Magnetic beads (22#:NE) | 1 | Analysis buffer | 1×20mL |
Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
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INCREMENT SERVICES
Magazine | Citations |
Renal Failure | Circulating Levels of Renalase, Norepinephrine, and Dopamine in Dialysis Patients Pubmed: 23530612 |
Acta Physiologica | Apelin-13 and APJ in paraventricular nucleus contribute to hypertension via sympathetic activation and vasopressin release in spontaneously hypertensive rats Pubmed:24995933 |
Physiological Research | Renal Sympathetic Denervation Improves Cardiac Dysfunction PubMed: 25804096 |
J Cardiovasc Pharmacol Ther | Erythropoietin Reverses Sepsis-Induced Vasoplegia to Norepinephrine Through Preservation of 伪1D-Adrenoceptor mRNA Expression and Inhibition of GRK2-Mediated Desensitization in Mouse Aorta. PubMed: 26025460 |
Neuropsychiatr Dis Treat | Catha edulis chewing effects on treatment of paranoid schizophrenic patients PubMed: 25926735 |
Journal of Caediovascular Pharmacology and Therapeutics | Erythropoietin Reverses Sepsis-Induced Vasoplegia to Norepinephrine Through Preservation of α1D-Adrenoceptor mRNA Expression and Inhibition of GRK2-Mediated Desensitization in Mouse Aorta content:21 |
Neuropharmacology | Relaxin in paraventricular nucleus contributes to sympathetic overdrive and hypertension via PI3K-Akt pathway Pubmed:26746861 |
Microchemical Journal | Determination of 17β-estradiol and noradrenaline in dog serum using surface-enhanced Raman spectroscopy and random Forest science:S0026265X16300388 |
Scientific Reports | Reduced lipolysis response to adipose afferent reflex involved in impaired activation of adrenoceptor-cAMP-PKA-hormone sensitive lipase pathway in obesity pubmed:27694818 |
PLoS One. | Artepillin C, a Typical Brazilian Propolis-Derived Component, Induces Brown-Like Adipocyte Formation in C3H10T1/2 Cells, Primary Inguinal White Adipose Tissue-Derived Adipocytes, and Mice. pubmed:27598888 |
Cellular Physiology and Biochemistry | Renal Denervation Attenuates Multi-Organ Fibrosis and Improves Vascular Remodeling in Rats with Transverse Aortic Constriction Induced Cardiomyopathy pubmed:27889753 |
European Journal of Trauma and Emergency | Xuebijing injection treatment inhibits vasopermeability and reduces fluid requirements in acanine burn model. pubmed:28070608 |
Acta Physiologica | c-Jun N-terminal Kinase mediates prostaglandin-induced sympathoexcitation in rats with chronic heart failure by reducing GAD1 and GABRA1 expression pubmed:27439062 |
Nutrition | Ginkgo biloba extract alleviates oxidative stress and some neurotransmitters changes induced by aluminum chloride in rats S0899900716302350 |
Auris Nasus Larynx. | Stress hormonal changes in the brain and plasma after acute noise exposure in mice. pubmed:27496010 |
Oncotarget | Effects of renal denervation on monocrotaline induced pulmonary remodeling pubmed:28187460 |
JOURNAL OF APPLIED PHYSIOLOGY | Renal sympathetic denervation attenuates hypertension and vascular remodeling in renovascular hypertensive rats pubmed:27742806 |
Nutrition | Ginkgo Biloba Extract Alleviates Oxidative Stress and Some Neurotransmitters Changes Induced by Aluminum Chloride in Rats pubmed:28241996 |
Acta Physiologica | Beneficial Effects of Renal Denervation on Cardiac Angiogenesis in Rats with Prolonged Pressure Overload pubmed:27575955 |
Proceedings of the National Academy of Sciences of the United States of America (PNAS) | cAMP-inducible coactivator CRTC3 attenuates brown adipose tissue thermogenesis Pubmed:29784793 |
Toxicology Reports | Neuro-and nephroprotective effect of grape seed proanthocyanidin extract against carboplatin and thalidomide through modulation of inflammation, tumor … Pubmed:29854627 |
International Journal of Nursing Practice | Non‐pharmacological interventions during childbirth for pain relief, anxiety, and neuroendocrine stress parameters: A randomized controlled trial Pubmed:29512230 |
Acta Astronautica | A novel sympathetic neuronal GABA ergic signaling system regulates NE release to prevent ventricular arrhythmias after acute myocardial infarction |
Journal of Taibah University for Science | Neurotoxicity and inflammation induced by individual and combined exposure to iron oxide nanoparticles and silver nanoparticles |
Journal of the American College of Cardiology | Noninvasive Stereotactic Radiotherapy for Renal Denervation in a Swine Model Pubmed: 31558254 |
American Journal of Physiology-Heart and Circulatory Physiology | Exacerbated pressor and sympatho-excitatory effects of central Elabela in spontaneously hypertensive rats Pubmed: 31834836 |
European Journal of Pharmacology | Resiniferatoxin reduces ventricular arrhythmias in heart failure via selectively blunting cardiac sympathetic afferent projection into spinal cord in rats Pubmed: 31811858 |
A porcine model for studying the cardiovascular consequences of high‐thoracic spinal cord injury Pubmed: 31876952 | |
The American Journal of Pathology | Sympathetic Nerves Positively Regulate Eosinophil-Driven Allergic Conjunctivitis via α1-Adrenergic Receptor Signaling Pubmed: 32194050 |
pharmaceutical biology | Role of melatonin in intestinal mucosal injury induced by restraint stress in mice Pubmed: 32298156 |
Nature | Feeding induces cholesterol biosynthesis via the mTORC1–USP20–HMGCR axis Pubmed: 33177714 |
European Journal of Medical Research | Laparoscopic-based perivascular renal sympathetic nerve denervation: a feasibility study in a porcine model Pubmed: 32552871 |
Scientific Reports | Baroreceptor denervation reduces inflammatory status but worsens cardiovascular collapse during systemic inflammation Pubmed: 32332859 |
bioRxiv | Baroreceptor denervation reduces inflammatory status and worsens cardiovascular collapse during systemic inflammation |
Blockade of adrenergic β‐receptor activation through local delivery of propranolol from a 3D collagen/polyvinyl alcohol/hydroxyapatite scaffold promotes bone repair … | |
Cogent Medicine | Fractionation and bioassay-guided isolation of antihypertensive components of senecio serratuloides |
PHARMACOLOGICAL RESEARCH | Piezo2 channel in nodose ganglia neurons is essential in controlling hypertension in a pathway regulated directly by Nedd4-2 33352230 |
ACTA PHARMACOLOGICA SINICA | The vascular endothelial growth factor trap aflibercept induces vascular dysfunction and hypertension via attenuation of eNOS/NO signaling in mice 33303990 |
Mol Metab | Adipose tissue lipolysis is regulated by PAQR11 via altering protein stability of phosphodiesterase 4D 33549845 |
International Journal of?Nanomedicine | Water-Soluble Carbon Dots in Cigarette Mainstream Smoke: Their Properties and the Behavioural, Neuroendocrinological, and Neurotransmitter Changes They ¡ 33762821 |
Int J Hypertens | The Effects of Renal Nerve Denervation on Blood Pressure and Target Organs in Different Hypertensive Rat Models 33884205 |
Kidney Blood Press Res | Renal interstitial fibrosis is reduced in high-fat diet-induced obese pigs following renal denervation from the intima and adventitia of the renal artery 34852339 |
Hypertension | Expression of LHCGR (Luteinizing Hormone/Chorionic Gonadotrophin Receptor) in Pheochromocytomas Unveils an Endocrine Mechanism Connecting Pregnancy … Pubmed:35189708 |
Exp Eye Res | Autonomic nervous system receptor-mediated regulation of mast cell degranulation modulates the inflammation after corneal epithelial abrasion Pubmed:35421396 |
Catalog No. | Related products for research use of Pan-species (General) Organism species | Applications (RESEARCH USE ONLY!) |
CPA907Ge11 | BSA Conjugated Noradrenaline (NE) | Immunogen; SDS-PAGE; WB. |
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PAA907Ge01 | Polyclonal Antibody to Noradrenaline (NE) | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
LAA907Ge81 | FITC-Linked Polyclonal Antibody to Noradrenaline (NE) | WB; IHC; ICC; IF. |
MAA907Ge25 | Monoclonal Antibody to Noradrenaline (NE) | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
MAA907Ge24 | Monoclonal Antibody to Noradrenaline (NE) | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
MAA907Ge23 | Monoclonal Antibody to Noradrenaline (NE) | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
MAA907Ge21 | Monoclonal Antibody to Noradrenaline (NE) | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
CEA907Ge | ELISA Kit for Noradrenaline (NE) | Enzyme-linked immunosorbent assay for Antigen Detection. |
IEA907Ge | Instant ELISA Kit for Noradrenaline (NE) | Enzyme-linked immunosorbent assay for Antigen Detection. |
HEA907Ge | High Sensitive ELISA Kit for Noradrenaline (NE) | Enzyme-linked immunosorbent assay for Antigen Detection. |
MEA907Ge | Mini Samples ELISA Kit for Noradrenaline (NE) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA907Ge | Multiplex Assay Kit for Noradrenaline (NE) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
KSA907Ge11 | ELISA Kit DIY Materials for Noradrenaline (NE) | Main materials for "Do It (ELISA Kit) Yourself". |