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Multiplex Assay Kit for Cluster Of Differentiation 26 (CD26) ,etc. by FLIA (Flow Luminescence Immunoassay)
CD26; ADA; DPPIV; DPP-IV; DPP4; ADABP; ADCP2; TP103; Adenosine Deaminase Complexing Protein 2; T-cell activation antigen CD26; Dipeptidyl Peptidase IV
(Note: Up to 8-plex in one testing reaction)
- Product No.LMA884Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3.5h
- Detection Range0.78-800pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.26 pg/mL.
- DownloadInstruction Manual
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
- FOB
US$ 405
US$ 420
US$ 443
US$ 475
US$ 506
US$ 552
US$ 622
Result
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Specificity
This assay has high sensitivity and excellent specificity for detection of Cluster Of Differentiation 26 (CD26) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Cluster Of Differentiation 26 (CD26) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Cluster Of Differentiation 26 (CD26) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Cluster Of Differentiation 26 (CD26) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 93-101 | 97 |
EDTA plasma(n=5) | 95-102 | 98 |
heparin plasma(n=5) | 93-101 | 97 |
sodium citrate plasma(n=5) | 95-105 | 99 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cluster Of Differentiation 26 (CD26) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cluster Of Differentiation 26 (CD26) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cluster Of Differentiation 26 (CD26) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 79-102% | 78-94% | 78-99% | 80-104% |
EDTA plasma(n=5) | 82-98% | 96-105% | 94-102% | 95-103% |
heparin plasma(n=5) | 78-101% | 78-89% | 78-96% | 79-96% |
sodium citrate plasma(n=5) | 98-105% | 91-105% | 91-105% | 78-104% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
96-well plate | 1 | Plate sealer for 96 wells | 4 |
Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
Pre-Mixed Magnetic beads (22#:CD26) | 1 | Analysis buffer | 1×20mL |
Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
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Magazine | Citations |
hormone and metabolic research | Linagliptin Blocks Renal Damage in Type 1 Diabetic Rats by Suppressing Advanced Glycation End Products-Receptor Axis Pubmed: 24710699 |
Curr Med Chem. | MK-0626, a dipeptidyl peptidase-4 inhibitor, improves neovascularization by increasing both the number of circulating endothelial progenitor cells and endothelial nitric oxide synthetase expression. Pubmed:24059225 |
Diabetes | Ghrelin inhibition restores glucose homeostasis in hepatocyte nuclear factor-1alpha (MODY3) deficient mice PubMed: 25979074 |
The American Journal of the Medical Sciences | Expression and Clinical Significance of Serum Dipeptidyl Peptidase IV Chronic Obstructive Pulmonary Disease Pubmed:26992252 |
Gene | Expression of recombinant human α-lactalbumin in milk of transgenic cloned pigs is sufficient to enhance intestinal growth and weight gain of suckling piglets Pubmed:26899869 |
Applied In Vitro Toxicology | Hepatic Spheroids for Long-Term Toxicity Studies aivt.2016.0016 |
BMJ Open Diab Res Care | High circulating plasma dipeptidyl peptidase- 4 levels in non-obese Asian Indians with type 2 diabetes correlate with fasting insulin and LDL-C levels, triceps skinfolds, total intra-abdominal adipose tissue volume and presence of diabetes: a case–control study 10.1136:bmjdrc-2017-000393 |
Diabetology & Metabolic Syndrome | Dipeptidyl peptidase-4 levels are increased and partially related to body fat distribution in patients with familial partial lipodystrophy type 2 pubmed:28450900 |
Journal of Cellular Biochemistry | The effect of CD26‐deficiency on dipeptidyl peptidase 8 and 9 expression profiles in a mouse model of Crohn's disease Pubmed:29693275 |
Medicine (Baltimore) | Rapid detection of urinary soluble intercellular adhesion molecule-1 for determination of lupus nephritis activity Pubmed:29953010 |
Sci Rep | The novel adamantane derivatives as potential mediators of inflammation and neural plasticity in diabetes mice with cognitive impairment Pubmed:35468904 |
Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
RPA884Mu02 | Recombinant Cluster Of Differentiation 26 (CD26) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA884Mu01 | Recombinant Cluster Of Differentiation 26 (CD26) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA884Mu02 | Polyclonal Antibody to Cluster Of Differentiation 26 (CD26) | WB; IHC; ICC; IP. |
PAA884Mu01 | Polyclonal Antibody to Cluster Of Differentiation 26 (CD26) | WB; IHC; ICC; IP. |
LAA884Mu71 | Biotin-Linked Polyclonal Antibody to Cluster Of Differentiation 26 (CD26) | WB; IHC; ICC. |
SEA884Mu | ELISA Kit for Cluster Of Differentiation 26 (CD26) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA884Mu | Multiplex Assay Kit for Cluster Of Differentiation 26 (CD26) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |