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Multiplex Assay Kit for Heat Shock Protein 70 (HSP70) ,etc. by FLIA (Flow Luminescence Immunoassay)
DnaK; 70 Kilodalton Heat Shock Proteins
(Note: Up to 8-plex in one testing reaction)
- Product No.LMA873Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3.5h
- Detection Range0.2-200ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.067 ng/mL.
- DownloadInstruction Manual
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
- FOB
US$ 449
US$ 467
US$ 492
US$ 527
US$ 562
US$ 613
US$ 691
Result
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Specificity
This assay has high sensitivity and excellent specificity for detection of Heat Shock Protein 70 (HSP70) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Heat Shock Protein 70 (HSP70) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Heat Shock Protein 70 (HSP70) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Heat Shock Protein 70 (HSP70) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 90-101 | 97 |
EDTA plasma(n=5) | 89-103 | 96 |
heparin plasma(n=5) | 96-105 | 101 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Shock Protein 70 (HSP70) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Shock Protein 70 (HSP70) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Heat Shock Protein 70 (HSP70) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 79-98% | 97-104% | 80-105% | 88-104% |
EDTA plasma(n=5) | 78-90% | 92-105% | 96-104% | 91-99% |
heparin plasma(n=5) | 85-101% | 79-92% | 78-105% | 93-103% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
96-well plate | 1 | Plate sealer for 96 wells | 4 |
Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
Pre-Mixed Magnetic beads (22#:HSP70) | 1 | Analysis buffer | 1×20mL |
Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
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Magazine | Citations |
Journal of Molecular Medicine | Proteomic analysis reveals significant elevation of heat shock protein 70 in patients with chronic heart failure due to arrhythmogenic right ventricular cardiomyopathy SpringerLink: b8738j3571287425 |
Journal of Neuroinflammation | Neuroinflammation in autism spectrum disorders PubMed: PMC3549857 |
Eur Arch Psychiatry Clin Neurosci. | Luteal serum BDNF and HSP70 levels in women with premenstrual dysphoric disorder Pubmed: 23455589 |
Arch Bronconeumol | Concentraciones de proteínas de estrés térmico, L-arginina y dimetilarginina asimétrica en pacientes con síndrome de apnea obstructiva del sue PubMed: 26055465 |
Arch Bronconeumol | Heat Shock Proteins, PubMed: 26055465 |
Animal Science Journal | The effect of heat stress on gene expression and synthesis of heat‐shock and milk proteins in bovine mammary epithelial cells PubMed: 26467738 |
Transplant Proc | L-Alanyl-Glutamine Attenuates Oxidative Stress in Liver Transplantation Patients PubMed: 26518955 |
Plos One | Long-Term Effects of Induced Hypothermia on Local and Systemic Inflammation-Results from a Porcine Long-Term Trauma Model Pubmed:27144532 |
J Pak Med Assoc. | Elevated interictal serum HSP-70 levels as an indicator of neurodegeneration for chronic migraine mnstemps:33-1429777310 |
Egyptian Poultry Science Journal | STUDY THE RELATIONSHIP BETWEEN SELENIUM AND HEAT SHOCK PROTEINS UNDER HEAT STRESS FOR LOCAL SINAI CHICKENS STRAIN abstract:3d115259031 |
Physiological consequences for rainbow trout (Oncorhynchus mykiss) of short-term exposure to increased suspended solid load S0144860916301935 | |
Animal Science Journal | Prokaryotic expression of chicken interferon-γ fusion protein and its effect on expression ofpoultry heat shock protein 70 under heat stress. pubmed:27753175 |
Pharmacol Rep | High-dose testosterone enanthate supplementation boosts oxidative stress, but exerts little effect on the antioxidant barrier in sedentary adolescent male rat liver. pubmed:28531762 |
Biomed Pharmacother. | SIRT1 inhibits releases of HMGB1 and HSP70 from human umbilical vein endothelial cells caused by IL-6 and the serum from a preeclampsia patient and protects the cells from death. pubmed:28122311 |
Immunobiology | Concentrations of SP-A and HSP70 are associated with polarization of macrophages in pleural effusions of non-small cell lung cancer pubmed:29111316 |
Egyptian Poultry Science Journal | EFFECT OF DIETARY SOURCE OF SELENIUM ON PRODUCTIVE AND REPRODUCTIVE PERFORMANCE OF SINAI LAYING HENS UNDER HEAT STRESS CONDITIONS. 125788660 |
Biomedicine & Pharmacotherapy | SIRT1 inhibits releases of HMGB1 and HSP70 from human umbilical vein endothelial cells caused by IL-6 and the serum from a preeclampsia patient and protects the cells from death DOI:10.1016/j.biopha.2017.01.087 |
Egyptian Poultry Science Journal | IMPACT OF CHROMIUM PICOLINATE SUPPLEMENTATION ON PRODUCTIVE PERFORMANCE, IMMUNE RESPONSE AND HEAT SHOCK PROTEINS OF BROILER CHICKENS UNDER HEAT-STRESS CONDITION. ISSN: 1110-5623 |
Aquaculture | Physiological consequences of chronic exposure of rainbow trout (Oncorhynchus mykiss) to suspended solid load in recirculating aquaculture systems 10.1016/j.aquaculture.2017.11.030 |
Journal of dairy research | Detection of 70 kDa heat shock protein in the saliva of dairy cows. pubmed:28625184 |
Journal of animal science | Reducing rumen starch fermentation of wheat with three percent sodium hydroxide has the potential to ameliorate the effect of heat stress in grain-fed wethers pubmed:29293755 |
Biochemistry and Cell Biology | Ectopic expression of Miro 1 ameliorates seizures and inhibits hippocampal neurodegeneration in a mouse model of pilocarpine epilepsy Pubmed:29365285 |
Egyptian Poultry Science Journal | THE EFFECT OF CHROMIUM PICOLINATE SUPPLEMENTATION ON IMMUNE RESPONSE AND HEAT SHOCK PROTEINS OF LAYING HENS UNDER HEAT … article_5570_5c06907ac09a7724a58d2b796fe91aba.pdf |
Egyptian Poultry Science Journal | EFFECT OF MITIGATION OF HEAT STRESS BY EARLY HEATE ACCLIMATION AND GLUTAMINE INJECTION ON SOME PHYSIOLOGICAL MEASUREMENTS … : |
FASEB Journal | Signature-oriented investigation of the efficacy of multicomponent drugs against heart failure Pubmed: 30230922 |
Journal of Biological Sciences | Maged Mohamed Abd-Elhakim Barakat, Noha Ahmed El-Boghdady, Ebtehal Kamal Eldin Farrag, Ataa Abdelhalim Said and Sylvia Edward Shaker |
Biological Procedures Online | Effective Visualization and Easy Tracking of Extracellular Vesicles in Glioma Cells |
Journal of Biological Sciences | Protective and Curative Effects of Bombax ceiba Flower and Ziziphus spina christi Fruit Extracts on Gastric Ulcer |
博士论文 | PHYSIOLOGICAL ADAPTATIONS TO HEAT ACCLIMATION; REPERCUSSIONS ON CYCLING PERFORMANCE |
environmental science and pollution research | Possible prophylactic effect of omega-3 fatty acids on cadmium-induced neurotoxicity in rats' brains Pubmed: 31468353 |
ACTA TROPICA | Oxidative stress mediated apoptotic potential of mefloquine on experimental trichinellosis Pubmed: 33221280 |
BioMed Research International | Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress Pubmed: 32685488 |
Slovak Journal of Animal Science | EFFECTS OF HEAT STRESS ON PHYSIOLOGICAL PARAMETERS AND SERUM CONCENTRATION OF HSP70 IN INDIGENOUS BREEDS OF SHEEP IN NIGERIA |
博士论文 | Effect of organic acid blend and prebiotic on growth performance gut histomorphometry, tibia bone characteristics, reactive oxygen species, microbial count and … |
Translational Medicine Communications | Concurrent high-intensity aerobic and resistance exercise modulates systemic release of alarmins (HMGB1, S100A8/A9, HSP70) and inflammatory … |
Physiological impacts of suspended solids in recirculating aquaculture | |
Clin Oral Investig | Evaluation of heat shock protein 70 and toll-like receptor 4 expression in gingival crevicular fluid in response to orthodontic forces 34091792 |
Journal of Thermal Biology | Effects of L-proline on cellular responses of hen erythrocytes subjected to thermal stress 33627283 |
J Korean Neurosurg Soc | The Restorative Effect of Gallic Acid on the Experimental Sciatic Nerve Damage Model 34376039 |
PLoS One | Neuroprotective effect of sodium alginate against chromium-induced brain damage in rats Pubmed:35421180 |
Med Science | Identification of marker proteins in preterm and term birth: Relationship of rankl and HSP70 in the cord blood |
Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
SEA873Mu | ELISA Kit for Heat Shock Protein 70 (HSP70) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA873Mu | Multiplex Assay Kit for Heat Shock Protein 70 (HSP70) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |