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Reagent Preparation
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Multiplex Assay Kit for Coagulation Factor II (F2) ,etc. by FLIA (Flow Luminescence Immunoassay)
FII; TM; PT; Thrombin; Prothrombin; Pro-Thrombin; Activation peptide fragment 1; Activation peptide fragment 2
(Note: Up to 8-plex in one testing reaction)
- Product No.LMA820Bo
- Organism SpeciesBos taurus; Bovine (Cattle) Same name, Different species.
- Sample Typeserum
- Test MethodDouble-antibody Sandwich
- Assay Length3.5h
- Detection Range0.04-40ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.013 ng/mL.
- DownloadInstruction Manual
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
- FOB
US$ 498
US$ 517
US$ 546
US$ 584
US$ 623
US$ 680
US$ 766
Result
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Specificity
This assay has high sensitivity and excellent specificity for detection of Coagulation Factor II (F2) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Coagulation Factor II (F2) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Coagulation Factor II (F2) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Coagulation Factor II (F2) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 78-88 | 82 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Coagulation Factor II (F2) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Coagulation Factor II (F2) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Coagulation Factor II (F2) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 82-91% | 87-101% | 99-105% | 97-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
96-well plate | 1 | Plate sealer for 96 wells | 4 |
Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
Pre-Mixed Magnetic beads (22#:F2) | 1 | Analysis buffer | 1×20mL |
Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
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Blood Cells Mol Dis | Upregulation of cytokine signalling in platelets increases risk of thrombophilia in severe COVID-19 patients Pubmed:35180460 |
Catalog No. | Related products for research use of Bos taurus; Bovine (Cattle) Organism species | Applications (RESEARCH USE ONLY!) |
RPA820Bo01 | Recombinant Coagulation Factor II (F2) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA820Bo02 | Recombinant Coagulation Factor II (F2) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA820Bo01 | Polyclonal Antibody to Coagulation Factor II (F2) | WB; IHC; ICC; IP. |
SEA820Bo | ELISA Kit for Coagulation Factor II (F2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA820Bo | Multiplex Assay Kit for Coagulation Factor II (F2) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
KSA820Bo01 | ELISA Kit DIY Materials for Coagulation Factor II (F2) | Main materials for "Do It (ELISA Kit) Yourself". |