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Multiplex Assay Kit for Neuromedin B (NMB) ,etc. by FLIA (Flow Luminescence Immunoassay)

NM-B

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Neuromedin B (NMB) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Neuromedin B (NMB) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Neuromedin B (NMB) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Neuromedin B (NMB) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 96-105 101
EDTA plasma(n=5) 92-99 96
heparin plasma(n=5) 84-103 98

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuromedin B (NMB) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuromedin B (NMB) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Neuromedin B (NMB) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 82-103% 81-101% 97-105% 98-105%
EDTA plasma(n=5) 99-105% 95-102% 97-105% 80-91%
heparin plasma(n=5) 92-99% 99-105% 80-99% 81-104%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:NMB) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
    add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
Endocrinology Expression and regulation of neuromedin B in pituitary corticotrophs of male melanocortin 2 receptor-deficient mice Pubmed: 24742195
PLoS One Neuromedin B Restores Erectile Function by Protecting the Cavernous Body and the Nitrergic Nerves from Injury in a Diabetic Rat Model PubMed: 26207818
american journal of hematology Impact of natural Neuromedin‐B receptor variants on iron metabolism Pubmed: 31724192
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA803Mu01 Recombinant Neuromedin B (NMB) Positive Control; Immunogen; SDS-PAGE; WB.
RPA803Mu02 Recombinant Neuromedin B (NMB) Positive Control; Immunogen; SDS-PAGE; WB.
PAA803Mu01 Polyclonal Antibody to Neuromedin B (NMB) WB; IHC; ICC; IP.
PAA803Mu02 Polyclonal Antibody to Neuromedin B (NMB) WB; IHC; ICC; IP.
MAA803Mu22 Monoclonal Antibody to Neuromedin B (NMB) WB; IHC; ICC; IP.
CEA803Mu ELISA Kit for Neuromedin B (NMB) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA803Mu Multiplex Assay Kit for Neuromedin B (NMB) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.