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Multiplex Assay Kit for Cholecystokinin (CCK) ,etc. by FLIA (Flow Luminescence Immunoassay)
CCK-PZ
(Note: Up to 8-plex in one testing reaction)
- Product No.LMA802Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length1.5h
- Detection Range0.98-1000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.327 pg/mL.
- DownloadInstruction Manual
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
- FOB
US$ 393
US$ 408
US$ 431
US$ 461
US$ 491
US$ 537
US$ 605
Result
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Specificity
This assay has high sensitivity and excellent specificity for detection of Cholecystokinin (CCK) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Cholecystokinin (CCK) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Cholecystokinin (CCK) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Cholecystokinin (CCK) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 85-92 | 89 |
EDTA plasma(n=5) | 92-105 | 101 |
heparin plasma(n=5) | 86-101 | 95 |
sodium citrate plasma(n=5) | 95-103 | 99 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cholecystokinin (CCK) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cholecystokinin (CCK) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cholecystokinin (CCK) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 86-93% | 98-105% | 82-92% | 97-105% |
EDTA plasma(n=5) | 91-101% | 86-93% | 84-91% | 84-91% |
heparin plasma(n=5) | 88-102% | 97-104% | 89-96% | 86-93% |
sodium citrate plasma(n=5) | 92-105% | 80-105% | 96-103% | 94-103% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
96-well plate | 1 | Plate sealer for 96 wells | 4 |
Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
Pre-Mixed Magnetic beads (22#:CCK) | 1 | Analysis buffer | 1×20mL |
Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
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Magazine | Citations |
Journal of Obstetrics and Gynaecology Research? | Disturbed release of cholecystokinin in pregnant women with hyperemesis gravidarum Pubmed:25331205 |
World J Gastroenterol | Effects of sphincter of Oddi motility on the formation of cholesterol gallstones Pubmed:27350732 |
Journal of Ethnopharmacology | Luhong formula inhibits myocardial fibrosis in a paracrine manner by activating thegp130/JAK2/STAT3 pathway in cardiomyocytes. pubmed:28115285 |
Cellular physiology and biochemistry | Cholecystokinin Expression in the Development of Postinfarction Heart Failure. pubmed:29130944 |
JOURNAL OF PHARMACEUTICAL SCIENCES | Assessment of the role of cholecystokinin in hyperemesis gravidarum and correlation with its severity : |
Journal of Dairy Science | Effects of dietary leucine and phenylalanine on pancreas development, enzyme activity, and relative gene expression in milk-fed Holstein dairy calves Pubmed:29477524 |
Food and Nutrition Research | Alignments of endocrine, anthropometric, and metabolic parameters in type 2 diabetes after intervention with an Okinawa-based Nordic diet Pubmed:29599686 |
International Heart Journal | The Relationship of Appetite-Regulating Hormones in the Development of Cardiac Cachexia Pubmed: 30799377 |
Chinese Medicine | Hejie Zhitong prescription promotes sleep and inhibits nociceptive transmission-associated neurotransmitter activity in a rodent migraine model Pubmed: 33014123 |
foods | Appetite and Satiety Effects of the Acute and Regular Consumption of Green Coffee Phenols and Green Coffee Phenol/Oat β-Glucan Nutraceuticals in Subjects with … 34828792 |
npj Biofilms and Microbiomes | The microbiota-gut-kidney axis mediates host osmoregulation in a small desert mammal Pubmed:35379849 |
Food Funct | Digestion characteristics of quinoa, barley and mungbean proteins and the effects of their simulated gastrointestinal digests on CCK secretion in enteroendocrine STC … Pubmed:35587126 |
Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
RPA802Hu01 | Recombinant Cholecystokinin (CCK) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA802Hu01 | Polyclonal Antibody to Cholecystokinin (CCK) | WB; IHC; ICC; IP. |
MAA802Hu22 | Monoclonal Antibody to Cholecystokinin (CCK) | WB; IHC; ICC; IP. |
CEA802Hu | ELISA Kit for Cholecystokinin (CCK) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA802Hu | Multiplex Assay Kit for Cholecystokinin (CCK) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |