Packages (Simulation)
Reagent Preparation
Image (I)
Image (II)
Certificate
Multiplex Assay Kit for Toll Like Receptor 2 (TLR2) ,etc. by FLIA (Flow Luminescence Immunoassay)
CD282; TIL4; Toll/interleukin-1 receptor-like protein 4
(Note: Up to 8-plex in one testing reaction)
- Product No.LMA663Bo
- Organism SpeciesBos taurus; Bovine (Cattle) Same name, Different species.
- Sample TypeSaliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3.5h
- Detection Range0.02-20ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.007 ng/mL.
- DownloadInstruction Manual
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
- FOB
US$ 472
US$ 490
US$ 517
US$ 553
US$ 590
US$ 644
US$ 726
Result
For more details, please contact local distributors! US$ 907 Add to Price Calculator
Specificity
This assay has high sensitivity and excellent specificity for detection of Toll Like Receptor 2 (TLR2) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Toll Like Receptor 2 (TLR2) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Toll Like Receptor 2 (TLR2) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Toll Like Receptor 2 (TLR2) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 86-103 | 95 |
EDTA plasma(n=5) | 83-97 | 88 |
heparin plasma(n=5) | 98-105 | 102 |
sodium citrate plasma(n=5) | 88-105 | 91 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Toll Like Receptor 2 (TLR2) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Toll Like Receptor 2 (TLR2) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Toll Like Receptor 2 (TLR2) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 82-103% | 79-94% | 84-97% | 93-102% |
EDTA plasma(n=5) | 85-104% | 86-94% | 88-97% | 98-105% |
heparin plasma(n=5) | 97-105% | 97-104% | 83-101% | 85-94% |
sodium citrate plasma(n=5) | 78-93% | 97-105% | 78-105% | 85-102% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
96-well plate | 1 | Plate sealer for 96 wells | 4 |
Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
Pre-Mixed Magnetic beads (22#:TLR2) | 1 | Analysis buffer | 1×20mL |
Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
GIVEAWAYS
INCREMENT SERVICES
Magazine | Citations |
Chinese Medical Journal | Expression of soluble Toll-like receptors in pleural effusions CMJ: 2010823483999506297 |
PLoS One | Baicalein Reduces Airway Injury in Allergen and IL-13 Induced Airway Inflammation PubMed: PMC3639905 |
Clinical and Developmental Immunology | TLR2 in Pleural Fluid Is Modulated by Talc Particles during Pleurodesis PubMed: PMC3529880 |
Advances in Virology | The Possible Role of TLR2 in Chronic Hepatitis B Patients with Precore Mutation Pubmed: 24187552 |
J Matern Fetal Neonatal Med. | Cervical and vaginal fluid soluble Toll-like receptor 2 in pregnancies complicated by preterm prelabor rupture of membranes Pubmed:25028801 |
Respiratory Virology and Immunogenicity Advances in Experimental Medicine and Biology | Cytokines and Toll-Like Receptors in the Immune Response to Influenza Vaccination Pubmed:25248345 |
Iran J Immunol. | Correlation?between?salivary?Toll like receptor-2?concentration?and early childhood caries. Pubmed:25265998 |
Int J Dermatol. | Membrane and soluble Toll‐like receptor 2 in patients with psoriasis treated by Goeckerman therapy Pubmed:25266302 |
BMC Immunology | The discriminative capacity of soluble Toll-like receptor (sTLR)2 and sTLR4 in inflammatory diseases Pubmed:25406630 |
Pathol Biol (Paris) | Cathelicidin related antimicrobial peptide, laminin, Toll-like receptors and chemokines levels in experimental hypersensitivity pneumonitis in mice PubMed: 25834936 |
Plos One | Soluble Toll-Like Receptors 2 and 4 in Cerebrospinal Fluid of Patients with Acute Hydrocephalus followingAneurysmal Subarachnoid Haemorrhage. Pubmed:27223696 |
Open Forum Infect Dis | The Effect of Rosuvastatin on Markers of Immune Activation in Treatment-Naive Human Immunodeficiency Virus-Patients Pubmed:26835476 |
Perfusion | Pentraxin 3 and other inflammatory biomarkers related to atrial fibrillation in cardiac surgery pubmed:27852958 |
Journal For Neurovirology | Soluble TLR2 and 4 concentrations in cerebrospinal fluid in HIV/SIV-relatedneuropathological conditions. pubmed:27882497 |
Acta Orthopaedica | Toll-like receptors and their soluble forms differ in the knee and thumb basal osteoarthritic joints pubmed:28093922 |
Biological Markers and Guided Therapy | Features of the Pattern Recognition Receptor TLR-2 Expression and Cytokines IL-2, IL-4 Profile in Children with Food Hypersensitivity 10.12988:bmgt.2018.812 |
CHILD`S HEALTH | Prospective study on the significance of mucosal barrier dysfunction markers in assessing the effectiveness of the new strategy for treating food hypersensitivity in … 10.22141:2224-0551.13.2.2018.129548 |
Neurological Sciences | Vinpocetine regulates levels of circulating TLRs in Parkinson's disease patients Pubmed: 30315378 |
Biological Markers in Fundamental and Clinical Medicine | PROGNOSTIC ROLE OF IL-4, IL-2 AND TLR-2 FOR PREDICTING OF COURSE OF FOOD ALLERGY IN CHILDREN Doi: 10.29256/v.02.02.2018.escbm71 |
Biological Markers in Fundamental and Clinical Medicine | STUDY OF THE ACTION OF CHROMIUM-CONTAINING BIOPREPARATIONS FROM SPIRULINA ON INSULIN ACTIVITY IN RATS Doi: 10.29256/v.02.02.2018.escbm72 |
Biomedicine & Pharmacotherapy | Opuntioside, opuntiol and its metallic nanoparticles attenuate adjuvant-induced arthritis: Novel suppressors of Toll-like receptors-2 and-4 |
PLoS One | Aberrant DNA methylation of the toll-like receptors 2 and 6 genes in patients with obstructive sleep apnea Pubmed: 32069296 |
Georgian medical news | ASSOCIATIONS BETWEEN EFFICACY OF THE THERAPY AND CIRCADIAN FLUCTUATIONS OF ENDOTHELIAL NITRIC OXIDE SYNTHASE WITH TOLL-LIKE … Pubmed: 32242853 |
Medical and Clinical Chemistry | ДОбОвА вАРІАбЕЛЬНІСТЬ РІвНЯ РОзЧИННИХ ТОЛ-пОДІбНИХ РЕцЕпТОРІв 2 в СИРОвАТцІ КРОвІ ЖІНОК, ХвОРИХ НА РЕвМАТОїДНИй АРТРИТ: зв' … |
Sciences of Europe | FEATURES OF MYOCARDIAL REMODELING IN MEN WITH POST-INFARCTION CARDIOSCLEROSIS AND ITS RELATIONSHIP WITH TOLL-LIKE … |
CLINICAL INVESTIGATIONS | Levels of toll-like receptors 2 and 4 in serum of men with postinfarction cardiosclerosis and its relationship with antiphospholipid syndrome components |
Acta Obstet Gynecol Scand | A value of soluble Toll‐like receptor 2 and 4 in vaginally obtained amniotic fluid for the prediction of histological chorioamnionitis 34244993 |
Clinical and Translational Allergy | Altered innate immune profile in blood of systemic mastocytosis patients Pubmed:35734269 |
Catalog No. | Related products for research use of Bos taurus; Bovine (Cattle) Organism species | Applications (RESEARCH USE ONLY!) |
SEA663Bo | ELISA Kit for Toll Like Receptor 2 (TLR2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA663Bo | Multiplex Assay Kit for Toll Like Receptor 2 (TLR2) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |